| Project/Area Number |
15K20370
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kitasato University (2016)
Asahi University (2015) |
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Principal Investigator |
Hayashi Toru 北里大学, 医療衛生学部, 講師 (10454266)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | マイクロRNA / exosomes / 上皮間葉相互作用 / エピジェネティクス / 唾液腺 / 器官形成 |
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| Outline of Final Research Achievements |
MicroRNAs (miRNAs) are small RNAs that regulate gene expression. There is evidence that miRNAs are used as mobile signals for intercellular communication via extracellular vesicles including exosomes. Recently, we found that exosomal miRNAs released from the mesenchyme are mobile signals for epithelial-mesenchymal interactions during fetal submandibular gland (SMG) development.
Classic recombination experiments using SMG and mammary gland showed that the salivary mesenchyme is inductive to epithelium, suggesting that the SMG mesenchyme affect epithelial epigenetic states. Epigenetics are genomic modifications without alteration of the DNA sequences. We hypothesized that the exosomal miRNAs from SMG mesenchyme could be signals to induce changes in epigenetics during the classic recombination experiments. Our data suggested that epigenetics in SMG epithelium alter at embryonic day 15 and that some exosomal miRNAs could target a transcription factor important for mammary gland development.
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