Educidation of the innate immune response of odontoblasts against bacteria-derived factor in the pathogenesis of pulpitis

• Project/Area Number: 15K20406
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: TAKEGAWA Daisuke 徳島大学, 病院, 助教 (10632664)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 歯髄炎 / 象牙芽細胞 / 自然免疫 / インターフェロンγ / KN-3 細胞

Outline of Final Research Achievements

In the progression of pulpitis, marked infiltration of inflammatory cells such as activated T cells producing interferon-γ (IFN-γ) is observed. Odontoblasts located in the outermost layer of dental pulp first recognize caries-related pathogens, and have a capacity to produce various pro-inflammatory cytokines in response to microbial pattern recognition receptors.
This study demonstrated the synergistic effects of IFN-γ on cytokine production in KN-3 cells stimulated with bacteria-derived factors. These findings suggest that IFN-γ may modulate the innate immune response in odontoblasts.

Research Products

• [Journal Article] Functional roles of NOD1 in odontoblasts on dental pulp innate immunity. (2016)
  • Author(s): Hosokawa Y, Hirao K, Yumoto H, Washio A, Nakanishi T, Takegawa D, Kitamura C, Matsuo T
  • Journal Title: BioMed Research International Volume: 2016 Pages: 1-11
  • DOI: 10.1155/2016/9325436
  • NAID: 120006535016
  • Peer Reviewed / Open Access
• [Presentation] Interferon-γ Modulates the Innate Immune Response in Odontoblast-like Cells (2017)
  • Author(s): Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Takashi Matsuo
  • Organizer: IADR General Session 2017
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Effect of Interleukin-17A on CCL20 Production From Odontoblast-like Cells (2017)
  • Author(s): Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Takashi Matsuo
  • Organizer: IADR General Session 2017
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Anti-Inflammatory Effects of Polyphenols on Rat Odontblastic Cells (2017)
  • Author(s): Yuki. Hosokawa, Hiromichi Yumoto, Kouji Hirao, Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa, Takashi Matsuo
  • Organizer: IADR General Session 2017
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] 象牙芽細胞が有するMincleを介した死細胞認識の機構とその歯髄炎症反応における役割 (2015)
  • Author(s): 細川由樹、湯本浩通、平尾功治、中西正、武川大輔、松尾敬志
  • Organizer: 日本歯科保存学会 2015年度秋季学術大会(第143回)
  • Place of Presentation: 文京シビックホール（東京都文京区）
  • Year and Date: 2015-11-12
• [Presentation] Interleukin-17 がラット象牙芽細胞様細胞（KN-3）のCCL20産生に及ぼす影響 (2015)
  • Author(s): 中西正、武川大輔、平尾功治、湯本浩通、細川由樹、松尾敬志
  • Organizer: 日本歯科保存学会 2015年度秋季学術大会(第143回)
  • Place of Presentation: 文京シビックホール（東京都文京区）
  • Year and Date: 2015-11-12
• [Presentation] ラット象牙芽細胞（KN-3）に対するカテキンとカフェイン酸の抗炎症作用 (2015)
  • Author(s): 細川由樹、湯本浩通、平尾功治、中西正、武川大輔、松尾敬志
  • Organizer: 日本歯科保存学会 2015年度春季学術大会(第142回）
  • Place of Presentation: 北九州国際会議場（福岡県北九州市）
  • Year and Date: 2015-06-25
• [Presentation] ラット象牙芽細胞（KN-3）の自然免疫反応におけるシグナル伝達経路の解析 (2015)
  • Author(s): 平尾功治、湯本浩通、細川由樹、中西正、武川大輔、松尾敬
  • Organizer: 日本歯科保存学会 2015年度春季学術大会(第142回）
  • Place of Presentation: 北九州国際会議場（福岡県北九州市）
  • Year and Date: 2015-06-25