| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The purpose of this study was to investigate odontoblast induction and differentiation from iPS cells, and dentin formation using induced cells in vivo. To induce for odontoblast, embryonic bodies (EB) were formed from iPS cells and cultured with retinoic acid and BMP-4. It was suggested that inducted cells were odontoblasts by PCR and immunohistochemistry. It was shown that differentiation into odontoblasts significantly decreased in SOX11 knockdown group. Transplantation of induced odontoblasts into mice was formed bone-like hard tissues, however dentin formation was not clear. These results indicate that SOX11 is considered as an important upstream control gene in odontoblast differentiation.
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