Induction of odontoblast from iPS cells for novel dental restorative method.

• Project/Area Number: 15K20412
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: Nakajima Kei 東京歯科大学, 歯学部, 助教 (20733463)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: iPS細胞 / 分化誘導 / 象牙芽細胞 / 象牙質 / 再生医療

Outline of Final Research Achievements

The purpose of this study was to investigate odontoblast induction and differentiation from iPS cells, and dentin formation using induced cells in vivo. To induce for odontoblast, embryonic bodies (EB) were formed from iPS cells and cultured with retinoic acid and BMP-4. It was suggested that inducted cells were odontoblasts by PCR and immunohistochemistry. It was shown that differentiation into odontoblasts significantly decreased in SOX11 knockdown group. Transplantation of induced odontoblasts into mice was formed bone-like hard tissues, however dentin formation was not clear. These results indicate that SOX11 is considered as an important upstream control gene in odontoblast differentiation.

Research Products

• [Journal Article] Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium (2017)
  • Author(s): Tokita, R., Nakajima, K., Inoue, K., Al-Wahabi, A., Ser-Od, T., Matsuzaka, K., Inoue, T.
  • Journal Title: Dental Materials Journal Volume: 36 Issue: 1 Pages: 103-110
  • DOI: 10.4012/dmj.2016-087
  • NAID: 130005308610
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Effects of CO2 lasers on dental pulp biology in rats. (2016)
  • Author(s): Ser-Od, T., Yasumoto, M., Al-Wahabi, A., Nakajima, K., Murakami, S., Matsuzaka, K., Inoue, T.
  • Journal Title: Photomedicine and laser surgery Volume: 34 Issue: 4 Pages: 157-163
  • DOI: 10.1089/pho.2015.3997
  • Peer Reviewed
• [Journal Article] Dental pulp cells promote the expression of receptor activator of nuclear factor-κB ligand, prostaglandin E 2 and substance P in mechanically stressed periodontal ligament cells. (2016)
  • Author(s): Morikawa, T., Matsuzaka, K., Nakajima, K., Yasumura, T., Sueishi, K., Inoue, T.
  • Journal Title: Archives of Oral Biology Volume: 70 Pages: 158-164
  • DOI: 10.1016/j.archoralbio.2016.06.021
  • Peer Reviewed
• [Journal Article] Development of a functional biohybrid implant formed from periodontal tissue utilizing bioengineering technology. (2016)
  • Author(s): Nakajima K, Oshima M, Yamamoto N, Tanaka C, Koitabashi R, Inoue T, Tsuji T.
  • Journal Title: Tissue Engineering Part A. Volume: 22(17-18) Issue: 17-18 Pages: 1108-1115
  • DOI: 10.1089/ten.tea.2016.0130
  • Peer Reviewed / Open Access
• [Presentation] Application of tooth germ on alveolar bone regeneration. (2017)
  • Author(s): Nakajima, K., Inoue, K., Sumi, M., Akashi, Y., Matsuzaka, K., Inoue, T.
  • Organizer: 95th General Session & Exhibition of the IADR
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] iPS細胞分化誘導技術を応用した歯牙形成障害関連遺伝子の病態解析検査 (2016)
  • Author(s): 井上健児, 松坂賢一, 村上聡, 國分克寿, 中島啓, Tungalag Ser-Od, Akram Al-Wahabi, 明石良彦, 鷲見正美, 井上孝
  • Organizer: 第9回日本口腔検査学会総会・学術大会
  • Place of Presentation: 福島県郡山市
  • Year and Date: 2016-10-01