| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Because odontoblasts produce dentin matrix protein-1 (DMP-1), we examined whether the degraded products of DMP-1 by MMP-3 contribute to enhanced proliferation in mouse induced pluripotent stem (iPS) cells-derived odontoblast-like cells. IL-1β increased mRNA and protein levels of DMP-1 and extracellular matrix metalloproteinase inducer (Emmprin). The exogenous degraded products of DMP-1 by MMP-3 resulted in increased proliferation of odontoblast-like cells in a dose-dependent manner. Treatment with siRNA against MMP-3 and/or Emmprin potently suppressed the IL-1β-induced increase in DMP-1 expression and suppressed cell proliferation. Taken together, our current study demonstrates the sequential involvement of Emmprin, MMP-3, DMP-1 expression, and DMP-1 degradation products by MMP-3, in effecting IL-1β-induced proliferation of iPS cell-derived odontoblast-like cells.
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