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Development of cytokine, cell adhesion protein conjugate type DNA scaffold materials

Research Project

Project/Area Number 15K20420
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

Yamamoto Nana  福岡歯科大学, 口腔歯学部, 助教 (60736677)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsDNA / DNAジェリー / サイトカイン / 細胞接着タンパク質 / 歯根膜 / 間葉系幹細胞 / 細胞シート / 歯周病 / ジェリー / 骨補填材 / DNAスキャフォールド / 細胞接着タンパク質共役型
Outline of Final Research Achievements

We thought that utilize DNA as biomaterial. There is an osteoplasty promotion function, and DNA has the characteristic that it becomes Jerry of the porosity if mixed with water by adjusting quantity of addition because it is porous. DNA Jerry, and it was injectable. DNA specifically couples cationic protamine in the helical structure. Protamine which let cytokine and cell adhesion protein covalently link in a cross linker to DNA electrostatically, and DNA lets scaffold materials. It was difficult to establish the technique that a conjugaion. Examined a correlation of ingredient of the additive and new osteoplasty ability from in vitro, an in vivo examination, and DNA having the environment that promotes osteoplasty. The new osteoplasty ability the bone defect on the rat skull with DNA Jerry and recognized the new bone when observed it in microCT . The cytotoxicity was relatively mild when performed HE dyeing. It was suggested that use alone acted on predominance for new bone.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] Degradation rate of DNA scaffolds and bone regeneration2018

    • Author(s)
      Matsumoto Ayako、Kajiya Hiroshi、Yamamoto-M Nana、Yanagi Tsukasa、Imamura Ayaka、Okabe Koji、Fukushima Tadao、Kido Hirofumi、Ohno Jun
    • Journal Title

      J Biomed Mater Res B Appl Biomater.

      Volume: - Issue: 1 Pages: 122-128

    • DOI

      10.1002/jbm.b.34102

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salmon DNA Accelerates Bone Regeneration by Osteoblast Migration2017

    • Author(s)
      Sato A,Kajiya H,Mori N,Sato H,Kido H,Ohno J
    • Journal Title

      PLoS One

      Volume: 6 Pages: 1-15

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 低分解性のDNAスキャフォールドが骨再生に及ぼす効果2017

    • Author(s)
      松本彩子, 鍛治屋浩, 山本南奈, 柳束, 今村彩香, 藤崎誠一, 岡部幸司, 城戸寛史, 大野純.
    • Organizer
      福岡歯科大学学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 異なるDNAの長さによる細胞毒性と生体分離性について.2016

    • Author(s)
      森南奈, 松本彩子, 鍛治屋浩, 大野純, 坂上竜資, 福島忠男. 10月21-23日. (福岡市)
    • Organizer
      第23回日本歯科医学会
    • Place of Presentation
      福岡市
    • Year and Date
      2016-10-21
    • Related Report
      2016 Research-status Report
  • [Presentation] Effects of dyslipidemia through Toll like receptors on pre-osteoclasts.2016

    • Author(s)
      Ohgi K, Yoshinaga Y, Mori N, Nurakami H, Maruo N, Arita Y, Kawahara Y, Ryu T, Sakagami R.
    • Organizer
      第59回秋季日本歯周病学会
    • Place of Presentation
      新潟市
    • Year and Date
      2016-10-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 保存科新規Advanced OSCE課題「永久歯初期う蝕に対するフッ化物塗布」の評価2016

    • Author(s)
      畠山純子, 春名千英子, 松本典祥, 水上正彦, 廣松亮, 森南奈, 村上弘, 大城希美子, 吉永泰周, 松埼英津子, 泉利雄, 中牟田博敬, 佐藤博信, 坂上竜資, 阿南壽.
    • Organizer
      第35回日本歯科医学教育学会
    • Place of Presentation
      大阪府吹田市
    • Year and Date
      2016-06-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 幹細胞併用のためのDNAスキャフォールドの開発2016

    • Author(s)
      森 南奈、松本 彩子、鍛冶屋 浩、大城 希美子、吉永 泰周、村上 弘、廣松 亮、丸尾 直樹、有田 晴一、有田 陽一、河原 ゆり、笠 孝成、坂上 竜資、大野 純、福島 忠男
    • Organizer
      日本歯周病学会九州五大学日本臨床歯周病学会九州支部合同研修会
    • Place of Presentation
      長崎市
    • Related Report
      2016 Research-status Report
  • [Presentation] ケ白子由来DNAスキャフォールドの性質分析2016

    • Author(s)
      松本 彩子, 森 南奈, 鍛治屋 浩, 岡部 幸司, 城戸 寛史, 福島 忠男
    • Organizer
      第42回福岡歯科大学学会総会
    • Place of Presentation
      福岡市
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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