| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Based on the hypothesis that bone marrow derived cells in blood are indirectly involved in the control of local osteoclast differentiation and induction, we investigated the influence of supernatant of cultured BMSCs under various conditions on osteoclastogenesis, the expression of known osteoclast differentiation related factors expressed by BMSCs and we tried to identify the cell type that most expresses those factors. As a result, BMSCs cultured for a certain period on various surfaces had different properties on BMM differentiation to osteoclast. In addition, analysis of gene expression of BMSCs cultured on various surfaces by qPCR method confirmed that some cells in BMSCs produce factors that affect osteoclast differentiation.
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