New developmet of extremely effective salivaly marker for diagnosis of oral dryness

• Project/Area Number: 15K20456
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: The Nippon Dental University
• Principal Investigator: MIZUHASHI Fumi 日本歯科大学, 新潟生命歯学部, 准教授 (60386266)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔乾燥症 / Ca拮抗薬 / タンパク同定解析 / RNA抽出 / マーカー / iTRAQ法 / microRNA / マイクロアレイ解析 / シェーグレン症候群 / 唾液 / Ca 拮抗薬 / タンパク濃度 / 二次元電気泳動 / 銀染色

Outline of Final Research Achievements

Calcium antagonist are often used for the treatment of hypertension. There are many oral dryness patients who take the calcium antagonist. In this study, we investigated the specific protein and RNA to the oral dryness patients taking the calcium antagonist.
The results of this study was that the amount of salivary secretion decreased and the protein concentration increased in the oral dryness patients taking the calcium antagonist. The results of the global analysis of salivary protein in the oral dryness patients taking the calcium antagonist and healthy elderly subjects indicated that there are some specific proteins increasing or decreasing in the oral dryness patients taking the calcium antagonist.

Research Products

• [Journal Article] Oral dryness caused by calcium blocker -Comparison with saliva of healthy elderly persons and patients with Sjogren's syndrome- (2017)
  • Author(s): Fumi Mizuhashi, Kaoru Koide, Shuji Toya, Tomoko Nashida
  • Journal Title: Medical Research Archives Volume: 5 Pages: 1-12
  • Peer Reviewed / Open Access
• [Presentation] Ca拮抗薬による口腔乾燥症―健常高齢者とシェーグレン症候群患者の唾液との比較― (2017)
  • Author(s): 水橋 史，小出 馨，梨田智子，戸谷収二，近藤敦子，浅沼直樹，佐藤利英，渡會侑子，栗田 武
  • Organizer: 日本補綴歯科学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2017-06-30
• [Presentation] Ca拮抗薬による口腔乾燥症－健常高齢者とシェーグレン症候群患者の唾液との比較－ (2017)
  • Author(s): 水橋 史, 小出 馨, 梨田智子, 戸谷収二, 近藤敦子, 浅沼直樹, 佐藤利英, 渡會侑子, 栗田 武
  • Organizer: 第126回日本補綴歯科学会学術大会
• [Presentation] Analysis of protein ingredient in saliva of oral dryness patients caused by calcium blocker (2017)
  • Author(s): Mizuhashi F, Koide K, Nashida T, Toya S
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] Saliva of oral dryness patients taking calcium blocker and patients with Sjogren’s syndrome (2016)
  • Author(s): Fumi Mizuhashi, Kaoru Koide, Tomoko Nashida, Shuji Toya
  • Organizer: 日本歯科大学歯学会ウィンターミーティング
  • Place of Presentation: 日本歯科大学新潟生命歯学部