| Project/Area Number |
15K20476
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
Kato Hiroko 新潟大学, 医歯学系, 助教 (70749994)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 口腔粘膜 / 上皮細胞 / 再生医療 / 低酸素 / HIF-1α / 口腔粘膜上皮 / HIF-１α |
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| Outline of Final Research Achievements |
Oral mucosa keratinocytes cultured in hypoxia showed higher proliferation than control, whereas cells treated with DMOG or HIF-1α siRNA in hypoxic culture showed no difference in low calcium medium. Then we analyzed the relationship between HIF-1α expression level and maintenance of undifferentiation state observing the undifferentiation marker; α6 integrin by western blot. Cells cultured in hypoxia or treated with DMOG showed higher expression of α6 integrin than control, however, cells treated with HIF-1α siRNA in hypoxic culture showed about the same level with control. Therefore, it is indicated that HIF-1α expression might regulate the maintenance of undifferentiated state of oral mucosa keratinocytes.
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