Development of regenerative therapy on bone and nerve tissue using dental pulp stem cell

• Project/Area Number: 15K20494
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: HATA MASAKI 愛知学院大学, 歯学部, 講師 (20632871)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯髄幹細胞 / 細胞移植 / 顎骨欠損 / 糖尿病性神経障害

Outline of Final Research Achievements

In this study, we examined whether transplanted-DPSCs produce new bone and improve diabetic polyneuropathy. Rat-derived DPSCs were injected into a critical-sized bone on carvalial bone. The transplantation of DPSCs with Collagen showed bone formation starting from the edges as well as from the center of the defect. Human-derived DPSCs were injected into unilateral hindlimb skeletal muscles of streptozotocin-induced diabetic mouse. The transplantation of DPSCs improved the impaired sciatic nerve conduction velocity, sciatic nerve blood flow and sentation. These results revealed that DPSCs may be an effective regenerative therapy on bone and nerve tissue.

Research Products

• [Journal Article] Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells. (2015)
  • Author(s): Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K.
  • Journal Title: Stem Cell Research & Therapy Volume: 6 Issue: 1 Pages: 162-162
  • DOI: 10.1186/s13287-015-0156-4
  • Peer Reviewed / Open Access
• [Presentation] 糖尿病性神経障害に対するヒト歯髄幹細胞移植の有効性の検討 (2017)
  • Author(s): 秦 正樹，大見真衣子，小林泰子，中村信久，姫野龍仁，神谷英紀，尾澤昌悟，中村二郎，武部 純，松原達昭，成瀬桂子
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台国際センター 会議棟・展示棟（宮城県仙台市）
  • Year and Date: 2017-03-07
• [Presentation] ヒト歯髄幹細胞移植による糖尿病性神経障害に対する治療効果発現メカニズムの検討 (2017)
  • Author(s): 秦 正樹,大見真衣子,小林泰子,中村信久,宮部 愛,姫野龍仁,神谷英紀,尾澤昌悟,中村二郎,武部 純,松原達昭,成瀬桂子
  • Organizer: 第60回日本糖尿病学会年次学術集会
• [Presentation] EFFECTS OF DENTAL PULP STEM CELLS ON MAXILLOFACIAL BONE REGENERATION (2017)
  • Author(s): Hata Masaki, Matsukawa Ryohei, Aoyagi Atsushi, Omi Maiko, Matsuoka Ayumi, Ozawa Shogo, Naruse Keiko, Matsubara Tatsuaki, Takebe Jun
  • Organizer: 4th Joint meeting of the ISMR-AAMP CONFERENCE
  • Int'l Joint Research
• [Presentation] 磁場刺激を用いた歯髄幹細胞の骨組織再生治療への検討 (2016)
  • Author(s): 秦 正樹，大見真衣子，福澤 蘭，小島規永，成瀬桂子，尾澤昌悟， 松原達昭，武部 純
  • Organizer: 日本補綴歯科学会第125回学術大会
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-07-09
• [Presentation] 糖尿病性神経障害に対する歯髄細胞移植の応用 (2016)
  • Author(s): 秦 正樹，成瀬桂子，松原達昭
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場（大阪府・大阪市）
  • Year and Date: 2016-03-17
• [Presentation] 歯髄幹細胞の機能解析と組織再生治療への有効性の検討 (2015)
  • Author(s): 秦 正樹
  • Organizer: 第57回歯科基礎医学会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-11
• [Presentation] 骨補填材が歯髄幹細胞の増殖および骨分化に与える影響 (2015)
  • Author(s): 秦 正樹，大見真衣子，成瀬桂子，尾澤昌悟，松原達昭，田中貴信
  • Organizer: 第36回日本炎症・再生医学会
  • Place of Presentation: 虎ノ門ヒルズフォーラム（東京都・港区）
  • Year and Date: 2015-07-22