Development of method for using the growth factor releasing titanium and dental pulp stem cells for jaw bone reconstruction.

• Project/Area Number: 15K20500
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Yamagata University
• Principal Investigator: Yusa Kazuyuki 山形大学, 医学部, 医員 (80636960)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 顎顔面再建外科学 / 骨芽細胞 / 再生医学 / 分子生物学 / バイオマテリアル

Outline of Final Research Achievements

In this study, we tried to develop the bone regenerative therapy for jaw bone defect applied to zinc ion releasing titanium and dental pulp stem cells. To evaluated the stimulating effects of the zinc releasing titanium, we performed alkaline phosphatase activity, real time PCR, alizarin red staining.
Our results indicated the future possibility of clinical application of　zinc ion releasing titanium as a scaffold for jaw bone reconstruction.

Research Products

• [Journal Article] Eluted zinc ions stimulate osteoblast differentiation and mineralization in human dental pulp stem cells for bone tissue engineering. (2016)
  • Author(s): Yusa K, Yamamoto O, Iino M, Takano H, Fukuda M, Quao Z, Sugiyama T
  • Journal Title: Arch Oral Biol. Volume: 71 Pages: 162-169
  • DOI: 10.1016/j.archoralbio.2016.07.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro. (2016)
  • Author(s): Yusa K, Yamamoto O, Takano H, Fukuda M, Iino M.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 29462-29462
  • DOI: 10.1038/srep29462
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Disseminated Intravascular Coagulation after Surgery for Facial Injury. (2016)
  • Author(s): Ozaki H, Tachibana H, Ishikawa S, Yusa K, Kitabatake K, Iino M.
  • Journal Title: Case Rep Dent. Volume: 2016 Pages: 6053652-6053652
  • DOI: 10.1155/2016/6053652
  • Peer Reviewed / Open Access
• [Journal Article] Mandibular Reconstruction With Fibula Bone Graft Followed by Particulate Cancellous Bone and Marrow Graft With Titanium Mesh Tray. (2016)
  • Author(s): Ozaki H, Sakurai H, Yusa K, Kitabatake K, Kobayashi T, Iino M
  • Journal Title: J Oral Implantol. Volume: 42 Issue: 4 Pages: 381-384
  • DOI: 10.1563/aaid-joi-d-16-00009
  • Peer Reviewed / Open Access
• [Journal Article] Functional and aesthetic rehabilitation with maxillary prosthesis supported by two zygomatic implants for maxillary defect resulting from cancer ablative surgery: a case report/technique article. (2016)
  • Author(s): Ozaki H, Ishikawa S, Kitabatake K, Yusa K, Sakurai H, Iino M.
  • Journal Title: Odontology Volume: 104 Issue: 2 Pages: 233-238
  • DOI: 10.1007/s10266-015-0222-5
  • Peer Reviewed
• [Journal Article] Facilitation of bone resorption activities in synovial lavage fluid patients with mandibular condyle fractures. (2016)
  • Author(s): Takano H, Takahashi T, Nakata A, Nogami S, Yusa K, Kuwajima S, Yamazaki M, Fukuda M
  • Journal Title: J Oral Rehabil Volume: 43 Issue: 5 Pages: 1-1
  • DOI: 10.1111/joor.12388
  • Peer Reviewed
• [Journal Article] A case of lateral retropharyngeal node metastasis from squamous cell carcinoma of the tongue successfully treated with concurrent chemoradiotherapy （CCRT） (2016)
  • Author(s): 9.遊佐和之，山ノ内秀之，北畠健一朗，吉田雪絵，尾崎尚，橘寛彦，飯野光喜
  • Journal Title: Journal of Japanese Society of Oral Oncology Volume: 28 Issue: 2 Pages: 21-25
  • DOI: 10.5843/jsot.28.21
  • NAID: 130005158513
  • ISSN: 0915-5988, 1884-4995
  • Peer Reviewed
• [Journal Article] 外側咽頭後リンパ節転移に対し同時化学放射線療法が著効した舌扁平上皮癌の1例 (2016)
  • Author(s): 遊佐和之、山ノ内秀之、北畠健一朗、吉田雪絵、尾崎尚、橘寛彦、飯野光喜
  • Journal Title: 日本口腔腫瘍学会誌 Volume: 印刷中
  • NAID: 130005158513
  • Peer Reviewed
• [Journal Article] A Case of Simultaneous Unilateral Anterior and Posterior Stafne Bone Defects. (2016)
  • Author(s): Ozaki H, Ishikawa S, Kitabatake K, Yusa K, Tachibana H, Iino M
  • Journal Title: Case Repeports in Dentistry. Volume: 印刷中 Pages: 1-5
  • DOI: 10.1155/2015/983956
  • Peer Reviewed
• [Journal Article] Mandibular reconstruction with fibula bone graft followed by PCBM graft with titanium mesh tray. (2016)
  • Author(s): Ozaki H, Sakurai H, Yusa K, Kitabatake K, Kobayashi T, Iino M
  • Journal Title: Journal of oral implantology. Volume: 未定
  • Peer Reviewed
• [Journal Article] 下顎再建およびインプラント補綴に関する臨床的検討 (2015)
  • Author(s): 遊佐和之、飯野光喜
  • Journal Title: みちのく歯學會雑誌 Volume: 46 Pages: 20-21
  • Peer Reviewed
• [Presentation] 亜鉛修飾型チタンの歯髄幹細胞における骨芽細胞分化促進作用の検討 (2016)
  • Author(s): 遊佐和之，尾崎尚，髙野裕史，福田雅幸，飯野光喜
  • Organizer: 第20回日本顎顔面インプラント学会総会・学術大会
  • Place of Presentation: 東京 東京医科歯科大学M&Dタワー
  • Year and Date: 2016-12-03
• [Presentation] 歯髄幹細胞および亜鉛修飾型チタンを用いた骨再生療法に関する基礎的研究 (2016)
  • Author(s): 遊佐和之，髙野裕史，福田雅幸，尾崎尚，北畠健一朗，橘寛彦，石川恵生，飯野光喜
  • Organizer: 第61回日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉 幕張メッセ
  • Year and Date: 2016-11-25
• [Presentation] 亜鉛徐放型チタンスキャフォールドおよび歯髄幹細胞を用いた骨増生法開発に関する基礎的検討 (2016)
  • Author(s): 遊佐和之，山本修，髙野裕史，福田雅幸，飯野光喜
  • Organizer: 第46回日本口腔インプラント学会学術大会
  • Place of Presentation: 愛知 名古屋国際会議場
  • Year and Date: 2016-09-16
• [Presentation] 下顎再建後のインプラント治療に関する検討 (2016)
  • Author(s): 遊佐和之、山ノ内秀之、北畠健一朗、尾崎尚、石川恵生、橘寛彦、櫻井博理、飯野光喜
  • Organizer: 第34回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 神奈川県横浜市 開港記念会館
  • Year and Date: 2016-01-21
• [Presentation] 下顎切除後の下顎再建およびインプラント治療による咬合再建に関する臨床的検討（第二報） (2015)
  • Author(s): 遊佐和之、尾崎尚、山ノ内秀之、橘寛彦、櫻井博理、飯野光喜
  • Organizer: 第19回顎顔面インプラント学会総会・学術大会
  • Place of Presentation: 神奈川県横須賀市 ホテルメルキュール横須賀
  • Year and Date: 2015-11-28
• [Presentation] 下顎再建およびインプラント補綴に関する臨床的検討 (2015)
  • Author(s): 遊佐和之、飯野光喜
  • Organizer: 第68回 東北地区歯科医学会
  • Place of Presentation: 岩手県盛岡市 岩手県歯科医師会館
  • Year and Date: 2015-10-31
• [Presentation] 当科における広範囲顎骨支持型補綴の臨床的検討 (2015)
  • Author(s): 遊佐和之、山ノ内秀之、北畠健一朗、尾崎尚、石川恵生、橘寛彦、櫻井博理、飯野光喜
  • Organizer: 第60回 日本口腔外科学会総会・学術大会
  • Place of Presentation: 愛知県名古屋市 名古屋国際会議場
  • Year and Date: 2015-10-16