Elucidation of permeation, metastaticity acquisition mechanism due to the platelet in the tissue in oral squamous cell carcinoma

• Project/Area Number: 15K20510
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kanazawa University
• Principal Investigator: KITAHARA HIROKO 金沢大学, 附属病院, 医員 (70507053)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔扁平上皮癌 / 血小板 / 上皮間葉移行

Outline of Final Research Achievements

In order for cancer cells to metastasize and invade, it is important for cancer cells to develop epithelial mesenchymal transition (EMT) and to construct an immunosuppressive microenvironment around the tumor. In recent years, attention has been drawn that platelets in blood vessels coat cancer cells, induce EMT, and act to promote invasion, metastasis. In this study, we investigated the induction of epithelial mesenchymal transition by coculturing oral squamous cell carcinoma cell line and platelets. As a result, it is suggested that epithelial mesenchymal transition may occur. In addition, immunostaining of tissue specimens obtained from oral squamous cell carcinoma patients showed that tumors tended to have mesenchymal characteristics in cases with high invasiveness. And it is suggested that platelet aggregation may be caused around.

Research Products

• [Journal Article] Regulation of PD-L1 expression in a high-grade invasive human oral squamous cell carcinoma microenvironment. (2017)
  • Author(s): Hirai M, Kitahara H, Kobayashi Y, Kato K, Bou-Gharios G, Nakamura H, Kawashiri S.
  • Journal Title: Int J Oncol Volume: 50 Issue: 1 Pages: 41-48
  • DOI: 10.3892/ijo.2016.3785
  • NAID: 120005973600
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Eribulin sensitizes oral squamous cell carcinoma cells to cetuximab via induction of mesenchymal-to-epithelial transition. (2016)
  • Author(s): Kitahara H, Hirai M, Kato K, Bou-Gharios G, Nakamura H, Kawashiri S.
  • Journal Title: Oncol Rep Volume: 36 Issue: 6 Pages: 3139-3144
  • DOI: 10.3892/or.2016.5189
  • NAID: 120005973603
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Loss of epidermal growth factor receptor expression in oral squamous cell carcinoma is associated with invasiveness and epithelial-mesenchymal transition (2016)
  • Author(s): Ito Kimura, Hiroko Kitahara, Kazuhiro Ooi, Koroku Kato, Natsuyo Noguchi, Kunio Yoshizawa, Hiroyuki Nakamura, Shuichi Kawashiri
  • Journal Title: Oncology Letters Volume: 11 Issue: 1 Pages: 201-207
  • DOI: 10.3892/ol.2015.3833
  • NAID: 120005973602
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 口腔扁平上皮癌細胞のInvadopodia形成と癌関連線維芽細胞(CAF)との関連 (2016)
  • Author(s): 加藤広禄、宮澤広樹、加藤阿希、平井真理子、吉本泰祐、木村依世、北原寛子、野口夏代、中村博幸、川尻秀一
  • Organizer: 第61日本口腔外科学会総会学術大会
  • Place of Presentation: 幕張メッセ（千葉）
  • Year and Date: 2016-11-26
• [Presentation] 薬剤関連顎骨壊死（MRONJ）の早期発見における bone scan index の有用性 (2016)
  • Author(s): 野口夏代、平井真理子、加藤阿希、吉本泰祐、木村依世、北原寛子、大井一浩、加藤広禄、中村博幸、川尻秀一
  • Organizer: 第61日本口腔外科学会総会学術大会
  • Place of Presentation: 幕張メッセ（千葉）
  • Year and Date: 2016-11-25
• [Presentation] The regulation of PD-L1 expression at microenvironment of high grade invasive human oral squamous cell carcinoma (2016)
  • Author(s): M Hirai, H Kitahara, I Kimura, K kato, G Bou-gharios, H nakamura, S kawashiri
  • Organizer: 24th Biennial congress of the european association for cancer research
  • Place of Presentation: Manchester(UK)
  • Year and Date: 2016-07-09
  • Int'l Joint Research
• [Presentation] Eribulin differentiaes cetuximab resistant oral squamous cell carcinoma cells to sensitive by inducing mesenchymal-epithelial transition(MET) (2016)
  • Author(s): H Kitahara, M Hirai, H Nakamura, S kawashiri
  • Organizer: 24th Biennial congress of the european association for cancer research
  • Place of Presentation: Manchester(UK)
  • Year and Date: 2016-07-09
  • Int'l Joint Research
• [Presentation] エリブリンのセツキシマブ非感受性口腔癌細胞での作用機序 (2016)
  • Author(s): 北原寛子、中村博幸、加藤広禄、野口夏代、大井一浩、川尻秀一
  • Organizer: 第40回日本頭頸部癌学会
  • Place of Presentation: ソニックシティ（埼玉）
  • Year and Date: 2016-06-10
• [Presentation] セツキシマブ非感受性口腔癌細胞でのエリブリンの作用検討 (2016)
  • Author(s): 北原寛子、平井真理子、中村博幸、川尻秀一
  • Organizer: 第70回日本口腔科学会
  • Place of Presentation: 福岡国際会議場（福岡）
  • Year and Date: 2016-04-17
• [Presentation] 口腔扁平上皮癌におけるPD-L1発現の検討 (2016)
  • Author(s): 平井真理子、北原寛子、中村博幸、川尻秀一
  • Organizer: 第70回日本口腔科学会
  • Place of Presentation: 福岡国際会議場（福岡）
  • Year and Date: 2016-04-16
• [Presentation] セツキシマブ非感受性口腔癌細胞でのエリブリンの作用検討 (2015)
  • Author(s): 北原寛子、木村依世、中村博幸、川尻秀一
  • Organizer: 第60回日本口腔外科学会総会学術大会
  • Place of Presentation: 名古屋国際会議場（愛知）
  • Year and Date: 2015-10-16
• [Presentation] セツキシマブ非感受性口腔癌の解析と新しい治療法の検討 (2015)
  • Author(s): 中村博幸、北原寛子、木村依世、川尻秀一
  • Organizer: 第60回日本口腔外科学会総会学術大会
  • Place of Presentation: 名古屋国際会議場（愛知）
  • Year and Date: 2015-10-16
• [Presentation] Loss of EGFR expression in oral squamous cell carcinoma is associated with invasiveness and epithelial-mesenchymal transition (2015)
  • Author(s): H Nakamura, I Kimura, H KItahara, A Kawashiri
  • Organizer: European Cancer Congress 2015
  • Place of Presentation: Vienna (Austria)
  • Year and Date: 2015-09-25
  • Int'l Joint Research
• [Presentation] Loss of EGFR expression in oral squamous cell carcinoma is associated with invasiveness and epithelial-mesenchymal transition (2015)
  • Author(s): I Kimura, H Kitahara, H Nakamura, S Kawashiri
  • Organizer: The Joint Meeting of 4th Congress go Asian Society of Head and Neck Oncology(ASHNO) and 39th Annual Meeting of Japan Society for Head and Neck Cancer(JSHNC)
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-06-03
  • Int'l Joint Research