| Project/Area Number |
15K20511
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Gifu University |
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Principal Investigator |
IIDA KAZUKI 岐阜大学, 大学院医学系研究科, 助教 (30585237)
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| Research Collaborator |
KAWAGUCHI tomoko
SUGIYAMA ken
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 歯髄細胞 / Sox11 / 骨分化 / iPS細胞 |
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| Outline of Final Research Achievements |
DPCs (dental pulp cells) derived from elder patients were difficult to be used in a regenerative medicine, because of their low capacities of differentiation and induction to iPS cells. We thought gene introduction of Sox11, a key gene, to DPCs enhanced their capacities of differentiation and induction to iPS cells. Sox11 did not enhanced differentiation capacity to osteoblast lineages, but nerve lineages. This suggest Sox11 may help DPCs to be nerve cells. And, Sox11 did not enhanced induction efficiency to iPS cells.
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| Academic Significance and Societal Importance of the Research Achievements |
DPCｓ(歯髄細胞）は、他の細胞へ変化する分化能力や万能細胞であるiPS細胞への高効率に変換する能力をもっているが、これは20代までの若年者のみであり、有病率の高い高齢者ではこれらの能力は低く、改善策が必要である。今回の研究ではkey geneとしてSox11に注目し、DPCsに遺伝子導入して、分化能やiPS誘導効率が向上するか解析した。結果として、Sox11はDPCｓを神経細胞分化させるのに有用であることがわかり、神経再生などの研究や治療に役立つ可能性があることがわかった。
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