Prospects of osteogenesis using adipose-derived MSCfat (ASC) and Fibrin seat.

• Project/Area Number: 15K20527
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: Matsubara Masakazu 岡山大学, 医歯薬学総合研究科, 助教 (50736551)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨再生 / 再生医療 / 脂肪幹細胞

Outline of Final Research Achievements

Dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) have been reported to show the similar multilineage potential with bone marrow-derived mesenchymal stem cells (MSCs), and can be more easily harvested from mature adipose tissue than MSCs. ASCs are heterogenous cell populations of mesodermal stem cells with contamination by smooth muscle cells, blood cells, perithelial cells, and endothelial cells in particular at early passage. Compared to ASCs, DFATs can be dedifferentiated from mature adipocytes to fibroblast-like cells in vitro and show high proliferative and high homogeneous. Bone tissue engineering of DFATs and ASCs is expected for the regenerative medicine. The purpose of this study was to compare the osteogenic potent of DFATs and ASCs.

Research Products

• [Journal Article] Stage IV sporadic Burkitt's leukaemia with osteolysis in the maxillary sinuses. (2016)
  • Author(s): Yoshioka Y, Yamachika E, Matsubara M, Iida S
  • Journal Title: Journal of surgical case reports Volume: 2 Issue: 2 Pages: 1-3
  • DOI: 10.1093/jscr/rjw011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Anti-osteoporosis effects of 1,4-dihydroxy-2-naphthoic acid in ovariectomized mice with increasing of bone density (2016)
  • Author(s): Kenichiro Kita, Eiki Yamachika, Masakazu Matsubara, Hidetsugu Tsujigiwa, Nobuhisa Ishida, Norifumi Moritani, Tatsushi Matsumura, Masahide Mizutani, Yuki Fujita, Kiyofumi Takabatake, Kenichiro Ejima, Hitoshi Nagatsuka, Yoshinori Yamaguchi, Seiji Iida
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 28(1) Issue: 1 Pages: 66-72
  • DOI: 10.1016/j.ajoms.2015.07.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Treatment of Osteonecrosis of the Jaw. (2015)
  • Author(s): Yamachika E, Matsubara M, Ikeda A, Matsumura T, Moritani N, Iida S.
  • Journal Title: Journal of Craniofacial Surgery Volume: 27(7) Issue: 7 Pages: 575-577
  • DOI: 10.1097/scs.0000000000002127
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 脱分化脂肪細胞による骨再生医療の展望 (2017)
  • Author(s): 松原正和
  • Organizer: 第62回日本口腔外科学会総会
• [Presentation] ウェアラブルカメラを用いた歯科臨床教育 (2016)
  • Author(s): 松原正和
  • Organizer: 第35回日本歯科教育学会
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-01
• [Presentation] 口腔粘膜欠損・歯槽骨欠損に対する自己血由来フィブリンポリマーの有用性 (2015)
  • Author(s): 松原 正和
  • Organizer: 第60回日本口腔外科学会 総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-10-16