| Project/Area Number |
15K20540
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Kotaro Ishii 九州大学, 大学病院, 助教 (60596386)
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| Research Collaborator |
SUGIURA Tsuyoshi 鹿児島大学, 顎顔面疾患制御学分野, 教授 (40322292)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 口腔癌 / 転移機構 / 腺様嚢胞癌 / 転移細胞株 / miRNA |
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| Outline of Final Research Achievements |
1. We established a lymph node metastatic subline from a highly metastatic AdCC cell line. DNA microarray hybridization and scanning were performed to compare RNA expression in lymph node metastatic cells with highly metastaticcells. The expression level of NNMT (Nicotinamide N-methyltransferase ) was up-regulated by 2000 times in lymph node metastatic cells. NNMT is known as a tumorigenesis agent. It was suggests that NNMT is involved in the metastasis in the AdCCs.
2.We performed miRNA microarray to identify differential miRNAs in serum pools of 6 OSCC patients and 6 health control. Then, we analyzed expression levels of miRNA in each serum sample of OSCC patients and control by real time PCR. MiR-183 showed significant over expression in patient group and was concerned with metastasis.
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