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Investigation of the mechanisms underlying chemoresistance through microRNA modulation and applications to individualized therapy in oral squamous cell carcinoma

Research Project

Project/Area Number 15K20546
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionKumamoto University

Principal Investigator

Kawahara Kenta  熊本大学, 医学部附属病院, 医員 (90732735)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords口腔癌 / microRNA / 抗癌剤耐性 / exosomal microRNA / exosomal miRNA / OSCC / Dicer / Drosha / miR-30a
Outline of Final Research Achievements

The purpose of this study is to determine the functional role of the miRNA which is involved in 5-FU resistance in oral squamous cell carcinoma (OSCC). The human OSCC, and the 5-FU-resistant cell lines were used in this study. Then, on the basis of the miRNA microarray data that miR-30a is significantly upregulated in the resistant cell lines, we investigated the effects of miR-30a on 5-FU sensitivity. We produced two miR-30a overexpressing stable cell lines, and confirmed that both of them showed increased 5-FU resistance.
Blood samples were collected before the administration of preoperative chemoradiotherapy for a routine laboratory analysis of the full blood count. Low expression of miR-1290 was significantly correlated with poor prognosis.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2016 2015

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] Pre-treatment neutrophil to lymphocyte ratio predicts the chemoradiotherapy outcome and survival in patients with oral squamous cell carcinoma2016

    • Author(s)
      Hikaru Nakashima, Yuichiro Matsuoka, Ryoji Yoshida, Masashi Nagata, Akiyuki Hirosue, Kenta Kawahara, Jyunki Sakata, Hidetaka Arita, Akimitsu Hiraki and Hideki Nakayama
    • Journal Title

      BMC Cancer

      Volume: 26 Issue: 1 Pages: 41-41

    • DOI

      10.1186/s12885-016-2079-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] IL-6 controls resistance to radiation by suppressing oxidative stress via the Nrf2-antioxidant pathway in oral squamous cell carcinoma.2016

    • Author(s)
      Matsuoka Yuichiro
    • Journal Title

      Br J Cancer

      Volume: 115 Issue: 10 Pages: 1234-1244

    • DOI

      10.1038/bjc.2016.327

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of active MMP release and invasive activity of lymph node metastatic tongue cancer cells by elevated signaling via the TNF-α-TNFR1-NF-κB pathway and a possible involvement of angiopoietin-like 4 in lung metastasis.2016

    • Author(s)
      Tanaka, T., Imamura, T., Yoneda, M., Irie, A., Ogi, H., Nagata, M., Yoshida, R., Fukuma, D., Kawahara, K., Shinohara, M, & Nakayama, H.
    • Journal Title

      Int. J. Oncol.

      Volume: 49 Issue: 4 Pages: 1377-1384

    • DOI

      10.3892/ijo.2016.3653

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Factors affecting volume change of myocutaneous flaps in oral cancer.2016

    • Author(s)
      Hiraki Akimitsu
    • Journal Title

      Int J Oral Maxillofac Surg

      Volume: 45 Issue: 11 Pages: 1395-1399

    • DOI

      10.1016/j.ijom.2016.04.014

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sentinel lymph node biopsy reduces the incidence of secondary neck metastasis in patients with oral squamous cell carcinoma.2016

    • Author(s)
      Hiraki Akimitsu
    • Journal Title

      Mol Clin Oncol

      Volume: 5 Pages: 57-60

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pre-treatment neutrophil to lymphocyte ratio predicts the chemoradiotherapy outcome and survival in patients with oral squamous cell carcinoma: a retrospective study.2016

    • Author(s)
      Nakashima H, Matsuoka Y, Yoshida R, Nagata M, Hirosue A, Kawahara K, Sakata J, Arita H, Hiraki A, Nakayama H.
    • Journal Title

      BMC Cancer

      Volume: 16

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] BRD4はMMP2のエピジェネティックな調節を介して口腔扁平上皮癌の転移に関与する2016

    • Author(s)
      山本達郎
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Phospho-Nrf2 is a useful predict factor of the response to chemoradiotherapy in oral squamous cell carcinoma.2016

    • Author(s)
      Yuichiro Matsuoka
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Investigation of the clinical significance of circulating miRNA-1290 in oral squamous cell carcinoma.2016

    • Author(s)
      Hikaru Nakashima
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 肺転移巣に対するCetuximab併用化学療法が奏効した唾液腺導管癌の1例2016

    • Author(s)
      川原健太
    • Organizer
      日本頭頸部癌学会学術総会
    • Place of Presentation
      大宮ソニックシティ
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 口腔癌の化学放射線治療と唾液の変化に関する検討2016

    • Author(s)
      川原 健太
    • Organizer
      第34回 日本口腔腫瘍学会
    • Place of Presentation
      横浜開港記念会館
    • Year and Date
      2016-01-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 転移性口腔扁平上皮癌細胞の放射線耐性について2015

    • Author(s)
      川原 健太
    • Organizer
      第39回 頭頸部癌学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-06-04
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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