Investigation of the mechanisms underlying chemoresistance through microRNA modulation and applications to individualized therapy in oral squamous cell carcinoma
| Project/Area Number |
15K20546
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
Kawahara Kenta 熊本大学, 医学部附属病院, 医員 (90732735)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 口腔癌 / microRNA / 抗癌剤耐性 / exosomal microRNA / exosomal miRNA / OSCC / Dicer / Drosha / miR-30a |
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| Outline of Final Research Achievements |
The purpose of this study is to determine the functional role of the miRNA which is involved in 5-FU resistance in oral squamous cell carcinoma (OSCC). The human OSCC, and the 5-FU-resistant cell lines were used in this study. Then, on the basis of the miRNA microarray data that miR-30a is significantly upregulated in the resistant cell lines, we investigated the effects of miR-30a on 5-FU sensitivity. We produced two miR-30a overexpressing stable cell lines, and confirmed that both of them showed increased 5-FU resistance.
Blood samples were collected before the administration of preoperative chemoradiotherapy for a routine laboratory analysis of the full blood count. Low expression of miR-1290 was significantly correlated with poor prognosis.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Enhancement of active MMP release and invasive activity of lymph node metastatic tongue cancer cells by elevated signaling via the TNF-α-TNFR1-NF-κB pathway and a possible involvement of angiopoietin-like 4 in lung metastasis.2016
Author(s)
Tanaka, T., Imamura, T., Yoneda, M., Irie, A., Ogi, H., Nagata, M., Yoshida, R., Fukuma, D., Kawahara, K., Shinohara, M, & Nakayama, H.
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Journal Title
Int. J. Oncol.
Volume: 49
Issue: 4
Pages: 1377-1384
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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