Roles of mu- and delta-opioid receptor in regulation of cholinergic activity in nucleus accumbens

• Project/Area Number: 15K20563
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: AONO Yuri 日本大学, 松戸歯学部, 助手(専) (50508497)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ドパミン / アセチルコリン / 側坐核 / 微小透析法 / ラット / アセチルコリン受容体 / 脳微小透析法 / 薬理学

Outline of Final Research Achievements

Nucleus accumbens (NAc) in a terminal areas of mesolimbic dopaminergic neurons that project from the ventral tegmental area. NAc contains cholinergic neurons and animal experiments have shown that selective depletion of these neurons induces cognitive impairment, including memory deficits. Opioids are used to control persistent pain and amnesia is a recognized adverse effect. There are three subtypes of opioid receptors: mu, delta and kappa. Accumbal cholinergic neurons express delta and mu receptors that are thought to inhibit cholinergic activity. In present study, we investigated the roles of delta and mu receptors in the regulation of accumbal acetylcholine efflux of freely moving rats by in vivo microdialysis.

Research Products

• [Journal Article] Mechanisms of delta- and mu-opioid receptor agonist-induced increases in extracellular dopamine level in the nucleus accumbens of freely moving rats (2017)
  • Author(s): Tadashi Saigusa, Yuri Aono, John L Waddington
  • Journal Title: Journal of Oral Science Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In vivo neurochemical evidence that delta1-, delta2- and mu2-opioid receptors, but not mu1-opioid receptors, inhibit acetylcholine efflux in the nucleus accumbens of freely moving rats. (2016)
  • Author(s): Kiguchi Y, Aono Y, Watanabe Y, Yamamoto-Nemoto S, Shimizu K, Shimizu T, Kosuge Y, Waddington JL, Ishige K, Ito Y, Saigusa T.
  • Journal Title: European Journal of Pharmacology Volume: 789 Pages: 402-410
  • DOI: 10.1016/j.ejphar.2016.07.028
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Activation of GABAB receptors inhibits δ2 receptor-mediated accumbal dopamine efflux in rats (2017)
  • Author(s): 青野悠里，渡邉由梨子，木口友里，石川学，渋谷 鑛，三枝 禎
  • Organizer: 第90回日本薬理学会年会
  • Place of Presentation: 長崎ブリックホール，長崎県，長崎市
  • Year and Date: 2017-03-16
• [Presentation] δ1 and δ2 receptors subtypes exert an inhibitory role in regulation of acetylcholine efflux in the nucleus accumbens of freely moving rats (2016)
  • Author(s): Yuri Kiguchi, Yuri Aono, Yuriko Watanabe, Seiko Yamamoto, Kunihiko Shimizu, Yasuhiro Kosuge, Kumiko Ishige, Yoshihisa Ito and Tadashi Saigusa
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-09
• [Presentation] δ受容体サブタイプ刺激が誘発したラットの側坐核ドパミン放出に対するmuscimolの効果の特徴 (2016)
  • Author(s): 青野悠里，木口友里，渡邉由梨子，三枝 禎
  • Organizer: 第134回日本薬理学会関東部会
  • Place of Presentation: 国際医療福祉大学，栃木県，大田原市
• [Presentation] δ1, δ2 and μ2, but not μ1 receptors, exert an inhibitory role in regulation of acetylcholine efflux in the nucleus accumbens of freely moving rats (2016)
  • Author(s): Tadashi Saigusa and Yuri Aono
  • Organizer: 30th The international College of Neuropsychopharmacology (CINP)
  • Place of Presentation: Seoul，Korea
  • Int'l Joint Research
• [Presentation] Muscimol inhibits the deltrophin II-, but not DPDPE-, induced increase of dopamine efflux in the nucleus accumbens of freely moving rats (2016)
  • Author(s): 青野悠里，三枝 禎
  • Organizer: 第46日本神経精神薬理学会
  • Place of Presentation: Seoul，Korea
• [Presentation] Intra-accumbal infusion of endomorphin-1 and endomorphin-2 decreases accumbal acetylcholine efflux via μ2 receptors in the nucleus accumbens of freely moving rats. (2015)
  • Author(s): Yuri Aono and Tadashi Saigusa
  • Organizer: Neuroscience 2015
  • Place of Presentation: McCormick Place（Chicago USA）
  • Year and Date: 2015-10-16
  • Int'l Joint Research
• [Presentation] Intra-accumbal infusion of endomorphin-2 reduces acetylcholine efflux via μ2 receptors in the nucleus accumbens of freely moving rats (2015)
  • Author(s): Yuri Aono and Tadashi Saigusa
  • Organizer: 第45回日本神経精神薬理学会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-09-24