| Project/Area Number |
15K20573
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 顎関節 / 幹細胞 / 老化促進マウス / 胎生期ラベリング法 / 変形性顎関節症 |
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| Outline of Final Research Achievements |
Senescence-Accelerated Mouse mice (SAM), SAMP 8 spontaneously develops osteoarthritis of the osteoarthritis, and the divided cells are labeled by administering BrdU (5-bromo-2'-deoxyuridine) for a certain period, After examining the localization of cells (LRCs) that kept labels in the temporomandibular joint after the administration period (2, 3 months), it was localized in joint capsule and ligament joint disc. In addition, it was confirmed that fluorescent immuno double staining was carried out using LRCs using the mesenchymal stem cell marker STRO-1, CD 146 antibody. In the temporomandibular joint of SAMP 8, the cartilage surface roughened and morphology of the mandibular head was observed. The length of the mandibular branch decreased as compared with the control group SAMR1. From the TMJ model, STRO-1, CD 146 antibody-positive cells and LRCs significantly decreased compared with the control group.
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