| Project/Area Number |
15K20577
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Ｓｃｌｅｒａｘｉｓ / 歯根膜細胞 / メカノトランスダクション / Scleraxis / 歯根膜 |
|---|
| Outline of Final Research Achievements |
In this study, we applied tensile force to human PDL fibroblasts to analyze relationship between Scleraxis (Scx), a transcription factor for tendon/ligament cell lineages, and TGF-β/Smad signaling.Scx mRNA expression was significantly up-regulated at 3 h. We also detected activation of p-Smad3. Pretreatment with SB-431542 or SIS3 showed not only inhibition of phosphorylation of Smad3 in a dose-dependent manner but also down-regulation of Scx mRNA. In conclusion, up-regulation of Scx mRNA in response to tensile force is regulated via TGF-β/Smad signaling.
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