| Project/Area Number |
15K20579
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 再生歯胚 / IGF-I / エナメル結節 / FGF4 / マイクロアレイ |
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| Outline of Final Research Achievements |
We reported that the IGF-I treatment increased the size and cusp number of bioengineered teeth. However, the biological mechanisms of IGF-I on the bioengineered teeth morphology remain unclear. In this study, we examined the biological mechanisms underlying the effect of IGF-I on the bioengineered teeth morphology. Microarray analysis was performed using dental epithelial cells derived from tooth germs. The IGF-I treated dental epithelial cells expressed high level of various factors which are expressed during tooth development. Moreover, the proliferation and differentiation of the IGF-I treated dental epithelial cells were significantly upregulated. The number of the enamel knot increased in the IGF-I treated bioengineered teeth. These data suggest that IGF-I is involved in the biological mechanisms during tooth development.
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