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Development of novel caries suppression method targeting polymer binding domain of plaque constituting bacteria

Research Project

Project/Area Number 15K20591
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionOkayama University

Principal Investigator

Takashima Yukiko  岡山大学, 医歯薬学総合研究科, 助教 (30589768)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsStreptococcus mutans / グルカン結合タンパク / 齲蝕 / バイオインフォマティクス / gbpC 遺伝子 / バイオフィルム / 結合ドメイン / 唾液タンパク
Outline of Final Research Achievements

Oral bacteria bind to other bacterial organisms and molecular proteins for biofilm formation. Streptococcus mutans, implicated as the primary causative agent of dental caries in humans, produces multiple glucan-binding proteins (Gbps), which are considered to promote formation of biofilm on tooth surfaces. In our previous study, we used bioinformatics to identify the glucan-binding domain sequence DPTKTIF, which is located in the middle of the gbpC gene encoding GbpC. In the present study, we constructed anti-DPTKTIF serum, and examined its effects on biofilm formation and binding to saliva proteins. Biofilm formation by S. mutans in the presence of anti-DPTKTIF serum was lower than that in its absence. In addition, anti-DPTKTIF serum was shown to bind to lysozyme. Our results suggest that an anti-DPTKTIF agent may be useful for inhibition of S. mutans adherence to the pellicle on tooth surfaces, resulting in inhibition of biofilm formation.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Contributions of Streptococcus mutans Cnm and PA antigens to aggravation of non-alcoholic steatohepatitis in mice2016

    • Author(s)
      Naka S, Hatakeyama R, Takashima Y, Matsumoto-Nakano M, Nomura R, Nakano K.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 36886-36886

    • DOI

      10.1038/srep36886

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of the dextran-binding domain in the glucan-binding protein C of Streptococcus mutans.2015

    • Author(s)
      Takashima Y, Fujita K, Ardin AC, Nagayama K, Nomura R, Nakano K, Matsumoto-Nakano M.
    • Journal Title

      Journal of Applied Microbiology

      Volume: 119 Issue: 4 Pages: 1148-1157

    • DOI

      10.1111/jam.12895

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Glucan-binding domain in GbpC of Streptococcus mutans contributes to binding to type I collagen2016

    • Author(s)
      Yukiko Takashima, Yuko Morikawa, Kazuyo Fujita, and Michiyo Matsumoto-Nakano
    • Organizer
      94th General Session & Exihibition of the IADR
    • Place of Presentation
      Seoul, Republic of Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 本院小児医療センター開設後の当科における患者実態調査2016

    • Author(s)
      高島 由紀子、吉田 翔、森本 節代、吉田 衣里、角田 陽子、平野 慶子、稲葉 裕明、仲野 道代
    • Organizer
      第35回日本小児歯科学会中四国地方会
    • Place of Presentation
      岡山
    • Related Report
      2016 Annual Research Report
  • [Presentation] Binding mechanism of Streptococcus mutans glucan-binding domain of GbpC to saliva proteins.2015

    • Author(s)
      Yukiko Takashima, Yuko Morikawa, Kazuyo Fujita, Michiyo Matsumoto-Nakano
    • Organizer
      The 63rd Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-10-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Role of glucan-binding domain of GbpC in biofilm formation by Streptococcus mutans.2015

    • Author(s)
      Yukiko Takashima, Yuko Morikawa, Kazuyo Fujita, Michiyo Matsumoto-Nakano
    • Organizer
      The 25th Congress of the International Association of Paediatric Dentistry
    • Place of Presentation
      The Scottish Exhibition & Conference Centre (SECC)
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Streptococcus mutans のバイオフィルム形成におけるグルカン結合領域の役割2015

    • Author(s)
      高島 由紀子, 森川 優子, 藤田 一世, 仲野 道代
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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