Development of novel caries suppression method targeting polymer binding domain of plaque constituting bacteria
| Project/Area Number |
15K20591
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Streptococcus mutans / グルカン結合タンパク / 齲蝕 / バイオインフォマティクス / gbpC 遺伝子 / バイオフィルム / 結合ドメイン / 唾液タンパク |
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| Outline of Final Research Achievements |
Oral bacteria bind to other bacterial organisms and molecular proteins for biofilm formation. Streptococcus mutans, implicated as the primary causative agent of dental caries in humans, produces multiple glucan-binding proteins (Gbps), which are considered to promote formation of biofilm on tooth surfaces. In our previous study, we used bioinformatics to identify the glucan-binding domain sequence DPTKTIF, which is located in the middle of the gbpC gene encoding GbpC. In the present study, we constructed anti-DPTKTIF serum, and examined its effects on biofilm formation and binding to saliva proteins. Biofilm formation by S. mutans in the presence of anti-DPTKTIF serum was lower than that in its absence. In addition, anti-DPTKTIF serum was shown to bind to lysozyme. Our results suggest that an anti-DPTKTIF agent may be useful for inhibition of S. mutans adherence to the pellicle on tooth surfaces, resulting in inhibition of biofilm formation.
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Report
(3 results)
Research Products
(7 results)
