Role of MSX1 in osteo/odontogenic differentiation of human dental pulp stem cells
Project/Area Number |
15K20592
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Orthodontics/Pediatric dentistry
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Research Institution | Hiroshima University |
Principal Investigator |
GOTO NORIKO 広島大学, 病院(歯), 歯科診療医 (10735137)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | MSX1 / 歯髄幹細胞 / 骨分化 / SREBP2 / コレステロール合成 / アルカリフォスファターゼ / RUNX2 / siRNA / 象牙芽細胞分化 / DNAマイクロアレイ / 脂質代謝 |
Outline of Final Research Achievements |
When human dental pulp stem cells were exposed to osteogenesis-induction medium, runt-related transcription factor-2 (RUNX2), bone morphogenetic protein-2 (BMP2), alkaline phosphatase (ALPL) mRNA levels, as well as alkaline phosphatase activity, increased on days 4-12 and thereafter the matrix was calcified on day 14. However, knockdown of MSX1 with small interfering RNA abolished the induction of the osteoblast-related gene expression, alkaline phosphatase activity and calcification. Interestingly, DNA microarray and PCR analyses revealed that MSX1 knockdown induced the sterol regulatory element-binding protein 2 (SREBP2) transcriptional factor and its downstream target genes in the cholesterol-synthesis pathway. Inhibition of cholesterol synthesis enhances osteoblast differentiation of various mesenchymal cells. Thus, MSX1 may downregulate the cholesterol synthesis-related genes to ensure osteoblast differentiation of human dental pulp stem cells.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] DEC2 is a negative regulator for the proliferation and differentiation of chondrocyte lineage-committed mesenchymal stem cells2016
Author(s)
Sasamoto, T., Fujimoto, K., Kanawa, M., Kimura, J., Takeuchi, J., Harada, N., Goto, N., Kawamoto, T., Noshiro, M., Suardita, K., Tanne, K., Kato, Y.
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Journal Title
Int. J. Mol. Med
Volume: 印刷中
Issue: 3
Pages: 876-884
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells2015
Author(s)
Fujii, S. Fujimoto, K. Goto, N. Kanawa, M. Kawamoto, T. Pan, H. Srivatanakul, P. Rakdang, W. Pornprasitwech, J. Saskianti, T. Suardita, K. Nishimura, F. Kato, Y.
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Journal Title
Biomed Rep.
Volume: 3
Issue: 4
Pages: 566-572
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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