| Project/Area Number |
15K20606
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 歯根膜由来血管内皮前駆細胞 / 牽引 / Periodontal ligament / Myofibroblast / Endothelial cell / Differentiation |
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| Outline of Final Research Achievements |
In order to develop the novel accelerated orthodontic therapy by controlling the orthodontic force-induced remodeling of fibrous tissue in the periodontal ligament (PDL), we tried to identify key genes which control the ability of PDL-derived endothelial progenitor cells (EPCs) to synthesize fibrous tissue in response to the mechanical stress: we constructed spheroidal aggregation with the PDL-derived EPCs, which was subsequently embedded in type I collagen gel. Then, the mechanical force was loaded to the PDL-derived EPCs in type I collagen gel. However, we unexpectedly found that it was very difficult to extract the protein and mRNA from the PDL-derived EPCs in type I collagen gel. Therefore, we are trying to establish suitable experimental method for effective extraction of protein and mRNA from these cells in the type I collagen gel.
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