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Proteomics of salivary peptides involved in Black stain

Research Project

Project/Area Number 15K20612
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

MATSUDA KIE  日本歯科大学, 新潟生命歯学部, 非常勤講師 (10633646)

Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsブラックステイン / 唾液 / 歯周病菌 / PCR / 色素沈着 / 唾液ペプチド / 歯冠色 / 歯学 / マイクロアレイ / プロテオーム / 蛋白質
Outline of Final Research Achievements

This study searches for the association between black stain and periodontopathic bacteria prevalence. The aim of the present study was to clarify the relationship between the periodontopathic bacteria present in tooth discoloration. We found a positive relationship between superficial tooth color and the number of periodontopathic bacterial species(J Dent Sci Ther.2016). We search for salivary peptides related to stain in their tooth, we outsourced a quantification of protein expression using iTRAQ reagents for the purpose of a comprehensive search for saliva in having black stain. Currently, we are analyzing the analysis results and performing Western blotting based on the results, focusing on histatin in saliva. In the future, we aim to compile research results and publish them in international journals.

Academic Significance and Societal Importance of the Research Achievements

近年、歯の審美性への要求は強くなっている。食物由来の色素沈着に対しては、原因や歯磨剤などでの対処法が広く周知されているが、細菌由来の色素沈着に対しては、歯科医院で専門的な器具を用いて除去する必要があるため、小児患児においては特に困難なことがある。細菌由来の色素沈着の原因である歯周病菌への対応や食物由来の色素沈着がお口の中の唾液(つば)成分とどのようにかかわっているか解明されれば、患児の負担を減らせる可能性を模索した。

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] An Analysis of the Periodontopathic Bacteria Involved in Extrinsic Discoloration of Teeth2016

    • Author(s)
      Kie Matsuda, Natsuki Suwa, Hitomi Kikuchi, Tsuneo Sekimoto and Akane Imai
    • Journal Title

      Journal of Dental Science and Therapy

      Volume: 1(2) Pages: 1-4

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 歯の着色に影響を及ぼす歯周病原性菌について2016

    • Author(s)
      今井あかね、松田貴絵
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report 2015 Research-status Report
  • [Presentation] Talk Eye Liteを用いた眼球運動測定2016

    • Author(s)
      田中 聖至, 松田 貴絵, 小野 幸絵, 加藤 雄一, 鴨田 剛司, 小松崎 明, 関本 恒夫
    • Organizer
      日本歯科医学教育学会
    • Place of Presentation
      大阪大学コンベンションセンター
    • Year and Date
      2016-07-01
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2022-11-04  

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