| Project/Area Number |
15K20617
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Health Sciences University of Hokkaido (2016-2017)
Tokyo Medical and Dental University (2015) |
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Principal Investigator |
YAMADA AZUSA 北海道医療大学, 歯学部, 助教 (30708847)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 間葉系幹細胞 / 骨芽細胞分化 / 歯根膜細胞 |
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| Outline of Final Research Achievements |
To identify the mechanism of osteoinductive medium (OIM)-induced osteoblastic differentiation of human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs), whole transcriptome analysis and RT-PCR were performed. As a result,
EGF like domain multiple 6 (EGFL6) was suggested to be involved in osteoblastic differentiation of hPDL-MSCs. Transfection of siEGFL6 significantly up-regulated the ALP activity of hPDL-MSCs in all samples. Knockdown of EGFL6 enhanced the expression of osteogenic genes, such as RUNX2, BSP, and OPN. Therefore, EGFL6 might be a novel regulator of osteogenesis in hPDL-MSCs.
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