Analysis of the effect of ketone bodies on bone metabolism

• Project/Area Number: 15K20628
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Showa University
• Principal Investigator: Saito Akihiro 昭和大学, 歯学部, 助教 (20736665)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ケトン体 / 骨芽細胞 / 骨代謝

Outline of Final Research Achievements

In this study, it was decided to investigate the mechanism of acetoacetate (AA) and b-hydroxybutyrate (HB) ALP activity / calcification formation regulation and its influence on osteoblast specific action.
It was found that AA and HB increased and suppressed ALP activity and calcification formation in a concentration-dependent and time-dependent manner in osteoblast-like cells MC3T3-E1 cells and primary osteoblasts.Furthermore, AA or HB was added to the medium during chondroblast-like cell ATDC5 cells and osteoclast-like cell RAW cell culture and the influence was analyzed. However, even when AA and HB were added, significance No change was seen.From this, it was found that AA and HB act on osteoblast specific.

Research Products

• [Journal Article] Enhanced and suppressed mineralization by acetoacetate andβ-hydroxybutyrate in osteoblast cultures. (2016)
  • Author(s): Saito A, Yoshimura K, Miyamoto Y, Yamamoto M, Kamijo R
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 473 Issue: 2 Pages: 537-544
  • DOI: 10.1016/j.bbrc.2016.03.109
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Remarks] 昭和大学歯学部歯周病学講座
  • URL: http://www10.showa-u.ac.jp/~perio/index.html
• [Remarks] 昭和大学歯学部口腔生化学講座
  • URL: http://www10.showa-u.ac.jp/~oralbio/