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Studies on outer membrane stability and permeability of primitive chloroplasts and its evolutionary relationships between Gram-negative bacteria

Research Project

Project/Area Number 15K20860
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Morphology/Structure
Research InstitutionTohoku University

Principal Investigator

KOJIMA Seiji  東北大学, 学際科学フロンティア研究所, 助教 (20745111)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞壁ペプチドグリカン / チャネル / 外膜 / グラム陰性細菌 / 葉緑体 / 灰色藻 / 藍藻 / 細胞壁 / ペプチドグリカン / 進化
Outline of Final Research Achievements

During the course of evolution from an endosymbiotic cyanobacterium to the chloroplast, the function of the outer membrane changed from permeability barrier to an interface that connects two metabolic entities, the chloroplast and host cell. In order to know how this functional alteration was possible, I studied on the outer membrane of primitive chloroplast (chloroplast of Cyanophora paradoxa).
I discovered two dominant proteins from the outer membrane of C. paradoxa chloroplast, and named them CppS and CppF. They function as non-specific diffusion channel. Surprisingly, their amino acid sequences showed no evolutionary linkage to cyanobacteria. My findings suggest that C. paradoxa chloroplast adopted the non-cyanobacterial proteins as its main outer membrane components, providing a diffusion route for various small substances across the outer membrane.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 2015 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Remarks (1 results)

  • [Journal Article] Outer membrane proteins derived from non-cyanobacterial lineage cover the peptidoglycan of Cyanophora paradoxa cyanelles and serve as a cyanelle diffusion channel.2016

    • Author(s)
      Kojima S, Muramoto K, Kusano T
    • Journal Title

      J. Biol. Chem.

      Volume: 291 Issue: 38 Pages: 20198-20209

    • DOI

      10.1074/jbc.m116.746131

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Peptidoglycan-associated outer membrane protein Mep45 of rumen anaerobe Selenomonas ruminantium forms a non-specific diffusion pore via its C-terminal transmembrane domain.2016

    • Author(s)
      S. Kojima, K. Hayashi, S. Tochigi, T. Kusano, J. Kaneko, Y. Kamio
    • Journal Title

      Biosci. Biochem. Biotechnol.

      Volume: 80 Issue: 10 Pages: 1954-1959

    • DOI

      10.1080/09168451.2016.1194185

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] グラム陰性細菌の多剤耐性― beta-lactam 系抗生物質の外膜透過・排出速度の測定結果を例に理解する―2016

    • Author(s)
      児島征司
    • Journal Title

      化学と生物

      Volume: 54 Pages: 80-82

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Bifunctional outer membrane protein of ruminal bacteria that acts as a diffusion channel via its C-terminal region and as an anchor binding to the peptidoglycan via its N-terminal region.2017

    • Author(s)
      Kojima S
    • Organizer
      Protein & Peptide conference-2017
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡)
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シアノバクテリア Synechocystis sp. PCC 6803 外膜の有機炭素及び無機イオン透過性の解析2017

    • Author(s)
      木幡光、栃木佐枝子、高橋秀幸、児島征司
    • Organizer
      日本農芸化学会 2017 年度大会
    • Place of Presentation
      京都女子大(京都)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Outer Membrane Permeability and Stability of Gram-negative Bacteria, in the Context of Multi-drug Resistance and Generation of Primitive Chloroplast.2016

    • Author(s)
      Kojima S
    • Organizer
      Thirteenth International Conference on Flow Dynamics
    • Place of Presentation
      仙台国際センター(仙台)
    • Year and Date
      2016-10-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] グラム陰性細菌及び原始的葉緑体の外膜の基本機能とその構造的安定性に関する研究2016

    • Author(s)
      児島征司
    • Organizer
      日本農芸化学会東北支部奨励賞受賞講演
    • Place of Presentation
      山形大学農学部(鶴岡)
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 偏性嫌気性細菌 Selenomonas ruminantium の主要外膜蛋白質 Mep45 は C 末端側 341 残基で拡散チャネルを形成する2016

    • Author(s)
      林華奈子、金子淳、栃木佐枝子、草野友延、神尾好是、児島征司
    • Organizer
      日本農芸化学会東北支部第 151 回大会
    • Place of Presentation
      山形大学農学部(鶴岡)
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 灰色藻の葉緑体外膜に存在する非藍藻系統由来の細胞壁ペプチドグリカン結合型外膜チャネル2016

    • Author(s)
      児島征司、村本光二、草野友延
    • Organizer
      日本植物学会第 80 回大会
    • Place of Presentation
      沖縄コンベンションセンター(宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Proteins originated from a non-cyanobacterial lineage constitute the dominant peptidoglycan-associated outer membrane protein in C. paradoxa cyanelle and serve as non-specific diffusion channel.2016

    • Author(s)
      Kojima S., K. Muramoto, and T. Kusano
    • Organizer
      Bacterial Cell Surfaces, Gordon Research Conferences
    • Place of Presentation
      West Dover, VT, USA
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 原始葉緑体が保持する細胞壁ペプチドグリカン結合型外膜チャネル2016

    • Author(s)
      児島征司、村本光二、草野友延
    • Organizer
      日本農芸化学会 2016 年度大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 灰色藻葉緑体が保持する N- アセチルプトレシン含有型細胞壁ペプチドグリカンに結合する蛋白質の単離と機能解析2015

    • Author(s)
      児島征司、村本光二、草野友延
    • Organizer
      日本ポリアミン学会第 7 回年会
    • Place of Presentation
      京都
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] 灰色藻葉緑体の細胞壁ペプチドグリカン結合蛋白質は非藍藻系統に由来する2015

    • Author(s)
      児島征司、村本光二、草野友延
    • Organizer
      日本植物学会第 79 回大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-08
    • Related Report
      2015 Research-status Report
  • [Remarks] グラム陰性細菌及び原始的葉緑体の外膜の基本機能とその構造的安定性に関する研究

    • URL

      http://www.ige.tohoku.ac.jp/tekio/wordpress/wp-content/themes/lsab_wp/img/Kojima_HP/kojima.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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