| Project/Area Number |
15K20876
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
Chemical biology
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| Research Institution | Tohoku University |
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Principal Investigator |
Tomoaki Ida 東北大学, 医学系研究科, 助教 (70570406)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 活性イオウ分子種 / 8-ニトロ-cGMP / システインパースルフィド / 酸化ストレス / 質量分析 / 活性システインパースルフィド / ポリスルフィド化タンパク質 / タンパク質ポリスルフィド化 / S-グアニル化 / ポリスルフィド |
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| Outline of Final Research Achievements |
We investigated novel oxidative stress response mechanism by 8-nitro-cGMP and reactive sulfur species such as cysteine persulfide. We developed a quantitative method, i.e, sulfur omics analysis, for determination of the level of cysteine persulfides/polysulfides and protein polysulfidation using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and iodoacetamide-based derivative. Using this technique, it was found that reactive sulfur species play an important role in oxidative stress response, via crosstalk with 8-nitro-cGMP. We also found a new cysteine persulfide producing system in cells, which is originally known as a master enzyme for protein translation, and elucidated its pivotal roles in protein polysulfidation and protein function.
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