Novel oxidative stress response mechanism by reactive sulfur species and 8-nitro-cGMP
Project/Area Number |
15K20876
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pathological medical chemistry
Chemical biology
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Research Institution | Tohoku University |
Principal Investigator |
Tomoaki Ida 東北大学, 医学系研究科, 助教 (70570406)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 活性イオウ分子種 / 8-ニトロ-cGMP / システインパースルフィド / 酸化ストレス / 質量分析 / 活性システインパースルフィド / ポリスルフィド化タンパク質 / タンパク質ポリスルフィド化 / S-グアニル化 / ポリスルフィド |
Outline of Final Research Achievements |
We investigated novel oxidative stress response mechanism by 8-nitro-cGMP and reactive sulfur species such as cysteine persulfide. We developed a quantitative method, i.e, sulfur omics analysis, for determination of the level of cysteine persulfides/polysulfides and protein polysulfidation using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and iodoacetamide-based derivative. Using this technique, it was found that reactive sulfur species play an important role in oxidative stress response, via crosstalk with 8-nitro-cGMP. We also found a new cysteine persulfide producing system in cells, which is originally known as a master enzyme for protein translation, and elucidated its pivotal roles in protein polysulfidation and protein function.
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Cysteine perthiosulfenic acid (Cys-SSOH): A novel intermediate in thiol-based redox signaling?2018
Author(s)
Heppner DE, Hristova M, Ida T, Mijuskovic A, Dustin CM, Bogdandi V, Fukuto JM, Dick TP, Nagy P, Li J, Akaike T, van der Vliet A.
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Journal Title
Redox Biol.
Volume: 14
Pages: 379-385
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Production of reactive persulfide species in chronic obstructive pulmonary disease2017
Author(s)
Numakura T, Sugiura H, Akaike T, Ida T, Fujii S, Koarai A, Yamada M, Onodera K, Hashimoto Y, Tanaka R, Sato K, Shishikura Y, Hirano T, Yanagisawa S, Fujino N, Okazaki T, Tamada T, Hoshikawa Y, Okada Y, Ichinose M
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Journal Title
Thorax
Volume: 72
Issue: 12
Pages: 1074-1083
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Synthesis and Characterization of 8-Nitroguanosine 3′,5′-Cyclic Monophosphorothioate Rp-Isomer as a Potent Inhibitor of Protein Kinase G1α2017
Author(s)
Ahmed KA, Zhang T, Ono K, Tsutsuki H, Ida T, Akashi S, Miyata K, Oike Y, Akaike T, Sawa T.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 40
Issue: 3
Pages: 365-374
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Protein polysulfidation-dependent persulfide dioxygenase activity of ethylmalonic encephalopathy protein 12016
Author(s)
Jung M, Kasamatsu S, Matsunaga T, Akashi S, Ono K, Nishimura A, Morita M, Abdul Hamid H, Fujii S, Kitamura H, Sawa T, Ida T, Motohashi H, Akaike T.
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Journal Title
Biochem Biophys Res Commun
Volume: 480
Issue: 2
Pages: 180-186
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Endogenous occurrence of protein S-guanylation in Escherichia coli: target identification and genetic regulation.2016
Author(s)
Tsutsuki, H., Jung, M., Zhang, T., Ono, K., Ida, T., Kunieda, K., Ihara, H., Akaike, T. and Sawa, T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 478
Issue: 1
Pages: 7-11
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Persistent activation of cGMP-dependent protein kinase by a nitrated cyclic nucleotide via site specific protein S-guanylation2016
Author(s)
Akashi S, Ahmed KA, Sawa T, Ono K, Tsutsuki H, Burgoyne JR, Ida T, Horio E, Prysyazhna O, Oike Y, Rahaman MM, Eaton P, Fujii S, Akaike T.
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Journal Title
Biochemistry
Volume: 55
Issue: 5
Pages: 751-761
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Thiosulfate mediates cytoprotective effects of hydrogen sulfide against neuronal ischemia2015
Author(s)
Marutani E, Yamada M, Ida T, Tokuda K, Ikeda K, Kai S, Shirozu K, Hayashida K, Kosugi S, Hanaoka K, Kaneki M, Akaike T, Ichinose F
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Journal Title
J Am Heart Assoc
Volume: 4
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] 8-Nitro-cGMP Enhances SNARE Complex Formation through S-Guanylation of Cys90 in SNAP252015
Author(s)
K. Kunieda, H. Tsutsuki, T. Ida, Y. Kishimoto, S. Kasamatsu, T. Sawa, N. Goshima, M. Itakura, M. Takahashi, T. Akaike, H. Ihara
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Journal Title
ACS Chemical Neuroscience
Volume: 6
Issue: 10
Pages: 1715-1725
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Hyperhomocysteinemia abrogates fasting-induced cardioprotection against ischemia/reperfusion by limiting bioavailability of hydrogen sulfide anions.2015
Author(s)
Nakano S, Ishii I, Shinmura K, Tamaki K, Hishiki T, Akahoshi N, Ida T, Nakanishi T, Kamata S, Kumagai Y, Akaike T, Fukuda K, Sano M, Suematsu M.
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Journal Title
J Mol Med (Berl).
Volume: 印刷中
Issue: 8
Pages: 879-889
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reactive sulfur species-mediated activation of the Keap1-Nrf2 pathway by 1,2-napthoquinone through sulfenic acids formation under oxidative stress.2015
Author(s)
Shinkai Y, Abiko Y, Ida T, Miura T, Kakehashi H, Ishii I, Nishida M, Sawa T, Akaike T, Kumagai Y.
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Journal Title
Chem Res Toxicol.
Volume: 印刷中
Issue: 5
Pages: 838-847
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Translation-coupled protein polysulfuration, a unique biosynthesis pathway of cysteine persulfide2016
Author(s)
Tomoaki Ida, Hideshi Ihara, Fanyan Wei, Kazuhito Tomizawa, Shingo Kasamatsu, Tetsuro Matsunaga, Yoshito Kumagai, Tomohiro Sawa, Hozumi Motohashi, Takaaki Akaike
Organizer
The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
Place of Presentation
Sendai International center(Aoba-ku, Sendai )
Year and Date
2016-05-20
Related Report
Int'l Joint Research
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[Book] レドックス疾患学2018
Author(s)
井田智章、西村 明、守田匡伸
Total Pages
276
Publisher
羊土社
ISBN
9784758103695
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