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Development of an epigenome-editing technology for inducing cancer epimutations in mice

Research Project

Project/Area Number 15K20888
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Tumor biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

ISEKI Hiroyoshi  筑波大学, 国際統合睡眠医科学研究機構, 助教 (50433652)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsエピゲノム編集 / CRISPR/Cas9 / lncRNA / がん / エピゲノム変異モデルマウス
Outline of Final Research Achievements

In this study, we attempted to develop an epigenome-editing technology by applying a CRISPR/Cas9 system and epigenome-modifying enzyme-scaffold proteins and lncRNAs. We first generated a functional small dCas9 mutant by deleting the N-terminal half of the dCas9 protein. Next, we developed an epigenome-editing system with epigenome-modifyling enzyme-scaffold proteins.We also identified a candidate epigenome-modifying enzyme-scaffold lncRNA.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2016

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Germline recombination in a novel Cre transgenic line, Prl3b1-cre mouse.2016

    • Author(s)
      Al-Soudy AS, Hiromori Y, Mizuno S, Hasegawa Y, Shawki HH, Katoh MC, Basha WA, Ibrahim AE, El-Shemy HA, Iseki Hi, Yoshiki A, Nagase H, Nakanishi T, Takahashi S, Oishi H, Sugiyama F.
    • Journal Title

      Genesis.

      Volume: 54(7) Issue: 7 Pages: 389-97

    • DOI

      10.1002/dvg.22944

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Generation of CRISPR/Cas9-mediated bicistronic knock-in <i>ins1-cre</i> driver mice2016

    • Author(s)
      Yoshikazu Hasegawa, Yoshikazu Hoshino, Abdelaziz E. Ibrahim, Kanako Kato, Yoko Daitoku1, Yoko Tanimoto, Yoshihisa Ikeda, Hisashi Oishi, Satoru Takahashi, Atsushi Yoshiki, Ken-ichi Yagami, Hiroyoshi Iseki, Seiya Mizuno, Fumihiro Sugiyama
    • Journal Title

      Experimental Animals

      Volume: 65 Issue: 3 Pages: 319-327

    • DOI

      10.1538/expanim.16-0016

    • NAID

      130006882371

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Combined overexpression of JARID2, PRDM14, ESRRB, and SALL4A dramatically improves efficiency and kinetics of reprogramming to induced pluripotent stem cells.2016

    • Author(s)
      Iseki H, Nakachi Y, Hishida T, Yamashita-Sugahara Y, Hirasaki M, Ueda A, Tanimoto Y, Iijima S, Sugiyama F, Yagami K, Takahashi S, Okuda A, Okazaki Y
    • Journal Title

      Stem Cells

      Volume: 34 Issue: 2 Pages: 322-333

    • DOI

      10.1002/stem.2243

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of a causative gene involved in resistance of lymphoma cells to rat parvovirus oncolytic infection by a genome-wide DNA methylation analysis2016

    • Author(s)
      伊関 大敬、今野 裕士、奥泉 久人、高橋 智、八神 健一、杉山 文博
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市西区みなとみらい一丁目1番1号 パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Jarid2過剰発現による効率的なiPS細胞誘導2016

    • Author(s)
      伊関 大敬、仲地 豊、菱田 友昭、菅原 泉、谷本 陽子、飯島 沙織、杉山 文博、八神 健一、高橋 智、奥田 晶彦、岡崎 康司
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都府京都市南区東九条下殿田町70番地 京都テルサ
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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