Development of an epigenome-editing technology for inducing cancer epimutations in mice
Project/Area Number |
15K20888
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory animal science
Tumor biology
|
Research Institution | University of Tsukuba |
Principal Investigator |
ISEKI Hiroyoshi 筑波大学, 国際統合睡眠医科学研究機構, 助教 (50433652)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | エピゲノム編集 / CRISPR/Cas9 / lncRNA / がん / エピゲノム変異モデルマウス |
Outline of Final Research Achievements |
In this study, we attempted to develop an epigenome-editing technology by applying a CRISPR/Cas9 system and epigenome-modifying enzyme-scaffold proteins and lncRNAs. We first generated a functional small dCas9 mutant by deleting the N-terminal half of the dCas9 protein. Next, we developed an epigenome-editing system with epigenome-modifyling enzyme-scaffold proteins.We also identified a candidate epigenome-modifying enzyme-scaffold lncRNA.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Germline recombination in a novel Cre transgenic line, Prl3b1-cre mouse.2016
Author(s)
Al-Soudy AS, Hiromori Y, Mizuno S, Hasegawa Y, Shawki HH, Katoh MC, Basha WA, Ibrahim AE, El-Shemy HA, Iseki Hi, Yoshiki A, Nagase H, Nakanishi T, Takahashi S, Oishi H, Sugiyama F.
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Journal Title
Genesis.
Volume: 54(7)
Issue: 7
Pages: 389-97
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Generation of CRISPR/Cas9-mediated bicistronic knock-in <i>ins1-cre</i> driver mice2016
Author(s)
Yoshikazu Hasegawa, Yoshikazu Hoshino, Abdelaziz E. Ibrahim, Kanako Kato, Yoko Daitoku1, Yoko Tanimoto, Yoshihisa Ikeda, Hisashi Oishi, Satoru Takahashi, Atsushi Yoshiki, Ken-ichi Yagami, Hiroyoshi Iseki, Seiya Mizuno, Fumihiro Sugiyama
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Journal Title
Experimental Animals
Volume: 65
Issue: 3
Pages: 319-327
DOI
NAID
ISSN
0007-5124, 1341-1357, 1881-7122
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Combined overexpression of JARID2, PRDM14, ESRRB, and SALL4A dramatically improves efficiency and kinetics of reprogramming to induced pluripotent stem cells.2016
Author(s)
Iseki H, Nakachi Y, Hishida T, Yamashita-Sugahara Y, Hirasaki M, Ueda A, Tanimoto Y, Iijima S, Sugiyama F, Yagami K, Takahashi S, Okuda A, Okazaki Y
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Journal Title
Stem Cells
Volume: 34
Issue: 2
Pages: 322-333
DOI
Related Report
Peer Reviewed
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