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The mechanism of compartmentalization of the cerebellar circuitry: an optogenetic approach using zebrafish

Research Project

Project/Area Number 15K20907
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Animal physiology/Animal behavior
General anatomy (including histology/embryology)
Research InstitutionSaitama University

Principal Investigator

TSUDA Sachiko  埼玉大学, 研究企画推進室, 助教 (80736786)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords小脳神経回路 / 区画形成 / ゼブラフィッシュ / 活動イメージング / 光遺伝学 / 膜電位センサー / カルシウムイメージング / 3次元的解析 / All optical system
Outline of Final Research Achievements

Although the general anatomical organization of the cerebellar neural circuitries is well defined, their functional development is still unresolved. To address this issue, especially focusing on cerebellar compartmentalization, we have applied optical approaches and behavior analysis to zebrafish. We conducted high-speed calcium imaging of neural activities of cerebellar Purkinje cells, which was combined with behavioral tests, such as optokinetic response (OKR) test and tactile stimulation. Specific populations of Purkinje cells were found to be activated during these tests, and their distribution patterns tend to differ along D-V axis. Furthermore, OKR was repressed by optogenetic stimulation targeting the activated regions. These findings suggest the structure and roles of the functional compartments in the developing cerebellum. We have also succeeded in detecting neuronal activities in zebrafish brain by using newly-developed voltage sensors, ASAP1.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Int'l Joint Research] Marine Biological Laboratory(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Underlying mechanism of the cyclic migrating motor complex in Suncus murinus a change in gastrointestinal pH is the key regulator2017

    • Author(s)
      Anupom Mondal, Kouhei Koyama, Takashi Mikami, Taichi Horita, Shota Takemi, Sachiko Tsuda, Ichiro Sakata & Takafumi Sakai
    • Journal Title

      Physiol Rep.

      Volume: 5(1) Issue: 1 Pages: e13105-e13105

    • DOI

      10.14814/phy2.13105

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Functional organization of cerebellar circuitry and its development2016

    • Author(s)
      Sachiko Tsuda
    • Organizer
      第22回国際動物学会第87回日本動物学会大会合同大会(口頭)
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県, 宜野湾市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamic migration of cell populations and functional compartmentalization in cerebellar development: 4D imaging in zebrafish2016

    • Author(s)
      Sachiko Tsuda
    • Organizer
      7th Asia Oceania Zebrafish Meeting(口頭)
    • Place of Presentation
      Singapore(Singapore)
    • Year and Date
      2016-10-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Morphological and functional analysis of compartmentalization in cerebellar development2016

    • Author(s)
      Sachiko Tsuda, Kanae Hiyoshi, Kazuhiro Maruyama, Hiroaki Miyazawa, Risa Kinno, Kyo Yamasu
    • Organizer
      第22回小型魚類研究会(ポスター)
    • Place of Presentation
      岡崎カンファレンスセンター(愛知県, 岡崎市)
    • Year and Date
      2016-08-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Structural and functional compartmentalization in cerebellar circuitry development: 4D imaging in zebrafish2016

    • Author(s)
      Sachiko Tsuda, Risa Kinno, Hiroaki Miyazawa, Kyo Yamasu
    • Organizer
      第39回日本神経科学大会(口頭)
    • Place of Presentation
      パシフィコ横浜(神奈川県, 横浜市)
    • Year and Date
      2016-07-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 小脳神経回路発生における形態・機能的な領域化:ゼブラフィッシュにおける4次元イメージング2016

    • Author(s)
      津田佐知子、金野李咲、宮澤碩彬、弥益恭
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 小脳機能的神経回路の形成機構:ゼブラフィッシュにおけるall-optogenetic system構築の試み2015

    • Author(s)
      津田佐知子、弥益恭
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-07-30
    • Related Report
      2015 Research-status Report
  • [Presentation] Dynamic migratory behaviors of gbx2-expressing cells during brain compartmentalization -4D imaging in zebrafish embryos-2015

    • Author(s)
      Sachiko Tsuda, Kyo Yamasu
    • Organizer
      48th Annual Meeting for the Japanese Society of Developmental Biologists and Asia-Pacific Developmental Biology Network
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2015-06-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] Molecular Neuroendocrinology: From Genome to Physiology2016

    • Author(s)
      Ryuichi Nakajima, Sachiko Tsuda, Jinsook Kim, George Augustine
    • Publisher
      Wiley-Blackwell
    • Related Report
      2016 Annual Research Report
  • [Book] Molecular Neuroendocrinology: From genometogenome to physiology2016

    • Author(s)
      Ryuichi Nakajima, Sachiko Tsuda, Jinsook Kim, George Augustine
    • Total Pages
      488
    • Publisher
      Wiley-Blackwell and the International Neuroendocrine Federation
    • Related Report
      2015 Research-status Report
  • [Remarks]

    • URL

      http://www.saitama-u.ac.jp/iron/tt/researchers/%E6%B4%A5%E7%94%B0-%E4%BD%90%E7%9F%A5%E5%AD%90

    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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