Molecular functions of sphingolipids in plants for technological development of metabolic engineering
| Project/Area Number |
15K20909
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
Applied molecular and cellular biology
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | スフィンゴ脂質 / スフィンゴリピドミクス / 細胞膜マイクロドメイン / ストレス耐性 / 代謝改変 / LC-MS/MS |
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| Outline of Final Research Achievements |
Plants have typical sphingolipid structures distinct from other eukaryotes. To dissect plant-specific structures of sphingolipids, we established a comprehensively targeted sphingolipidomics technology based on theoretical library covering whole molecular species present in plants. The technical development provided new insights into molecular functions of plant-specific sphingolipid structures in the formation of functional lipid-protein clusters as plasma membrane microdomains. Moreover, we succeeded to identify a novel glycosyl transferase gene family responsible for biosynthesis of the diverse sugar chains of plant sphingolipids. These results will further accelerate understanding of molecular functions of plant sphingolipids as well as applied biotechnological development through metabolic engineering of sphingolipids.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Loss of inositol phosphorylceramide sphingolipid mannosylation induces plant immune responses and reduces cellulose content in Arabidopsis.2016
Author(s)
Fang L, Ishikawa T, Rennie EA, Murawska GM, Lao J, Yan J, Tsai AY, Baidoo EE, Xu J, Keasling JD, Demura T, Kawai-Yamada M, Scheller HV, *Mortimer JC
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Journal Title
Plant Cell
Volume: 28
Issue: 12
Pages: 2991-3004
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] KONJAC1 and 2 are key factors for GDP-mannose generation and affect l-ascorbic acid and glucomannan biosynthesis in Arabidopsis.2015
Author(s)
Sawake S, Tajima N, Mortimer JC, Lao J, Ishikawa T, Yu X, Yamanashi Y, Yoshimi Y, Kawai-Yamada M, Dupree P, Tsumuraya Y and Kotake T
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Journal Title
Plant Cell
Volume: 27
Issue: 12
Pages: 3397-3409
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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