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Molecular functions of sphingolipids in plants for technological development of metabolic engineering

Research Project

Project/Area Number 15K20909
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Applied molecular and cellular biology
Research InstitutionSaitama University

Principal Investigator

ISHIKAWA Toshiki  埼玉大学, 理工学研究科, 助教 (20598247)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsスフィンゴ脂質 / スフィンゴリピドミクス / 細胞膜マイクロドメイン / ストレス耐性 / 代謝改変 / LC-MS/MS
Outline of Final Research Achievements

Plants have typical sphingolipid structures distinct from other eukaryotes. To dissect plant-specific structures of sphingolipids, we established a comprehensively targeted sphingolipidomics technology based on theoretical library covering whole molecular species present in plants. The technical development provided new insights into molecular functions of plant-specific sphingolipid structures in the formation of functional lipid-protein clusters as plasma membrane microdomains. Moreover, we succeeded to identify a novel glycosyl transferase gene family responsible for biosynthesis of the diverse sugar chains of plant sphingolipids. These results will further accelerate understanding of molecular functions of plant sphingolipids as well as applied biotechnological development through metabolic engineering of sphingolipids.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 2 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] Lawrence Berkeley National Laboratory(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Synthesis and degradation of long-chain base phosphates affect fumonisin B1-induced cell death in Arabidopsis thaliana.2017

    • Author(s)
      Yanagawa D, Ishikawa T, Imai H.
    • Journal Title

      J Plant Res

      Volume: 130 Issue: 3 Pages: 571-585

    • DOI

      10.1007/s10265-017-0923-7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and tageted quantitative profiling of the sphingolipidome in rice2016

    • Author(s)
      Ishikawa T, Ito Y, Kawai-Yamada M
    • Journal Title

      Plant J

      Volume: 88 Issue: 4 Pages: 681-693

    • DOI

      10.1111/tpj.13281

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Loss of inositol phosphorylceramide sphingolipid mannosylation induces plant immune responses and reduces cellulose content in Arabidopsis.2016

    • Author(s)
      Fang L, Ishikawa T, Rennie EA, Murawska GM, Lao J, Yan J, Tsai AY, Baidoo EE, Xu J, Keasling JD, Demura T, Kawai-Yamada M, Scheller HV, *Mortimer JC
    • Journal Title

      Plant Cell

      Volume: 28 Issue: 12 Pages: 2991-3004

    • DOI

      10.1105/tpc.16.00186

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plasma membrane microdomains are essential for Rac1-RbohB/H-mediated immunity in rice2016

    • Author(s)
      Nagan M, Ishikawa T, Fujiwara M, Fukao Y, Kawano Y, Kawai-Yamada M, Shimamoto K
    • Journal Title

      Plant Cell

      Volume: 28 Issue: 8 Pages: 1966-1983

    • DOI

      10.1105/tpc.16.00201

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of BAX INHIBITOR-1 links plasma membrane microdomain proteins to stress.2015

    • Author(s)
      Ishikawa T, Aki T, Yanagisawa S, Uchimiya H and Kawai-Yamada M
    • Journal Title

      Plant Physiol.

      Volume: 169 Issue: 2 Pages: 1333-1343

    • DOI

      10.1104/pp.15.00445

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] KONJAC1 and 2 are key factors for GDP-mannose generation and affect l-ascorbic acid and glucomannan biosynthesis in Arabidopsis.2015

    • Author(s)
      Sawake S, Tajima N, Mortimer JC, Lao J, Ishikawa T, Yu X, Yamanashi Y, Yoshimi Y, Kawai-Yamada M, Dupree P, Tsumuraya Y and Kotake T
    • Journal Title

      Plant Cell

      Volume: 27 Issue: 12 Pages: 3397-3409

    • DOI

      10.1105/tpc.15.00379

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] スフィンゴリピドミクスが解き明かす植物スフィンゴ脂質の独自性と多様性2016

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      第29回植物脂質シンポジウム
    • Place of Presentation
      大阪大学(大阪府豊中市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Comprehensively targeted sphingolipidomics reveals plant species- and lipid class-specific metabolism of sphingolipids in monocots2016

    • Author(s)
      Toshiki Ishikawa and Maki Kawai-Yamada
    • Organizer
      ISPL2016
    • Place of Presentation
      Goettingen, Germany
    • Year and Date
      2016-07-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物のストレス誘導性細胞死における細胞膜マイクロドメインの機能2016

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県・盛岡市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Plasma membrane microdomains participate in Bax inhibitor-1-mediated stress tolerance in rice.2015

    • Author(s)
      Ishikawa T and Kawai-Yamada M
    • Organizer
      The 6th Asian Symposium on Plant Lipids
    • Place of Presentation
      Singapore
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 植物スフィンゴリピドミクスによるストレス耐性分子マーカーの探索2015

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      第9回メタボロームシンポジウム
    • Place of Presentation
      三島商工会議所(静岡県・三島市)
    • Year and Date
      2015-10-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 植物スフィンゴ脂質の代謝と機能における 長鎖塩基不飽和化の役割2015

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      第28回植物脂質シンポジウム
    • Place of Presentation
      上智大学四ッ谷キャンパス(東京都)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Research-status Report
  • [Presentation] スフィンゴ脂質不飽和化酵素SLDを過剰発現するイネの解析2015

    • Author(s)
      石川寿樹、金松、中曽根光、長野稔、川合真紀
    • Organizer
      第33回日本植物細胞分子生物学会
    • Place of Presentation
      東京大学農学部キャンパス(東京都)
    • Year and Date
      2015-08-10
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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