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Development of new procedure to make chimeric animals without using naive pluripotent stem cells

Research Project

Project/Area Number 15K20948
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Laboratory animal science
Research InstitutionThe University of Tokyo

Principal Investigator

Masaki Hideki  東京大学, 医科学研究所, 助教 (20571988)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsES細胞 / iPS細胞 / 再生医療 / 臓器再生 / キメラ / 遺伝子改変動物 / ウサギ / 多能性幹細胞 / 臓器
Outline of Final Research Achievements

Our group had shown that forced expression of anti-apoptotic factor enables developmental stage mismatched cells, such as primed state pluripotent stem cells or endodermal progenitor cells, to form chimera with pre-implantation mouse embryos. In this research, we tried to clarify this approach is also applicable for non-rodent cells, and/or other lineage progenitor cells except for endodermal progenitor cells. As a result, we observed that rabbit ESCs formed chimera with mouse and rabbit pre-implantation embryo by forced expression of anti-apoptotic gene, whereas control rabbit ESCs did not form any chimeras. We also observed heart region specific chimera formation with Nkx2.5 expressing cardiomyocyte progenitor cells by preventing apoptosis. Therefore, we conclude that promotion of chimera formation via inhibition of apoptosis can be applicable not only for rodent, and not only for endodermal progenitors.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2016 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Interspecific in vitro assay for the chimera-forming ability of human pluripotent stem cells.2015

    • Author(s)
      Masaki H, Kato-Itoh M, Umino A, Sato H, Hamanaka S, Kobayashi T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H.
    • Journal Title

      Development.

      Volume: 142(18) Pages: 3222-30

    • DOI

      10.1242/dev.124016

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A UNIQUE SYSTEM TO ASSESS THE IN VIVO LINEAGE POTENTIALS OF DEVELOPMENTALLY-ADVANCED CELLS BY BLASTOCYST INJECTION2016

    • Author(s)
      Hideki Masaki
    • Organizer
      Annual Meeting of International Society for Stem Cell Research
    • Place of Presentation
      Moscone Center, San Francisco CA, USA
    • Year and Date
      2016-06-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Predict the unpredictable: using the in vivo niche to assess lineage potential and functionality of PSC-derived cells2016

    • Author(s)
      Hideki Masaki
    • Organizer
      Annual Meeting of International Society for Stem Cell Research
    • Place of Presentation
      Moscone Center, San Francisco CA, USA
    • Year and Date
      2016-06-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] A unique system to assess the in vivo lineage potentials of developmentally-advanced progenitors2016

    • Author(s)
      正木英樹
    • Organizer
      日本再生医療学会年会
    • Place of Presentation
      大阪国際展示場(大阪府大阪市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 幹細胞研究における生体環境利用の有用性2016

    • Author(s)
      正木英樹
    • Organizer
      日本再生医療学会
    • Place of Presentation
      大阪国際展示場(大阪府大阪市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Novel chimera forming technology by preventing cell death2015

    • Author(s)
      正木英樹
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-12-27  

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