Investigation of antimicrobial activity of hypothalamic peptides in chronic bacterial infection

• Project/Area Number: 15K21002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied health science; General internal medicine(including psychosomatic medicine)
• Research Institution: Niigata University
• Principal Investigator: TATEISHI Yoshitaka 新潟大学, 医歯学系, 准教授 (30433296)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 非結核性抗酸菌症 / グレリン / 抗菌活性 / 非結核性抗酸菌 / 抗炎症効果

Outline of Final Research Achievements

According to some previous reports and characteristics of cationic charge, we investigated whether ghrelin possesses antibiotic effect on non-tuberculous mycobacteria. We used two strains of non-tuberculous mycobacteria, Mycobacterium avium 104 as a reference strain and M. intracellulare as a hypervirulent clinical strain. Contrary to our expectation, the number of bacteria increased in ghrelin concentration-dependent manner both in direct incubation and in macrophage infection. Furthermore, culture supernatant of adipocytes and myocytes treated with ghrelin also increased the number of bacteria, which contradicts the indirect antibiotic effect by inhibiting catabolism by ghrelin. These data suggests that ghrelin does not possess antibiotic effect on non-tuberculous mycobacteria.

Research Products

• [Journal Article] Effects of nutritional and ambient oxygen condition on biofilm formation in Mycobacterium avium subsp. hominissuis via altered glycolipid expression (2017)
  • Author(s): Takahiro Totani, Yukiko Nishiuchi, Yoshitaka Tateishi, Yutaka Yoshida, Hiromi Kitanaka, Mamiko Niki, Yukihiro Kaneko, Sohkichi Matsumoto
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 41775-41775
  • DOI: 10.1038/srep41775
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 結核菌の病原因子 (2016)
  • Author(s): 立石善隆, 松本壮吉
  • Journal Title: 呼吸器内科 Volume: 29 Pages: 65-70
  • NAID: 40020719609
  • Peer Reviewed
• [Journal Article] 科学評論社 (2016)
  • Author(s): 立石善隆、松本壮吉
  • Journal Title: 呼吸器内科 Volume: 29 Pages: 65-70
• [Journal Article] 1.A New Screen for Tuberculosis Drug Candidates Utilizing a Luciferase-Expressing Recombinant Mycobacterium bovis Bacillus Calmette-Guéren. (2015)
  • Author(s): Ozeki Y, Igarashi M, Doe M, Tamaru A, Kinoshita N, Ogura Y, Iwamoto T, Sawa R, Umekita M, Enany S, Nishiuchi Y, Osada-Oka M, Hayashi T, Niki M, Tateishi Y, Hatano M, Matsumoto S.
  • Journal Title: PLoS One Volume: 10(11) Issue: 11 Pages: 1-28
  • DOI: 10.1371/journal.pone.0141658
  • Peer Reviewed / Open Access
• [Presentation] Role of nutritional richness, hypoxia, bioactive cell-wall glycopeptidolipid on biofilm formation in Mycobacterium avium subsp. hominissuis. (2016)
  • Author(s): Tateishi Y, Matsumoto S, Nishiuchi Y
  • Organizer: ASM microbe 2016
  • Place of Presentation: 米国ボストン
  • Year and Date: 2016-06-16
• [Presentation] Biofilm Formation in Mycobacterium avium subsp. hominissuis (2016)
  • Author(s): Yoshitaka Tateishi
  • Organizer: 第56回日本呼吸器学会総会
  • Place of Presentation: 京都国際会議場（京都市左京区）
  • Year and Date: 2016-04-08
• [Presentation] 結核菌におけるポリフェノールの抗菌作用の検討 (2016)
  • Author(s): 立石 善隆, 尾関 百合子, 西山 晃史, 松本 壮吉
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪市天王寺区）
  • Year and Date: 2016-03-23
• [Presentation] ルシフェラーゼ発現リコンビ;ナントBCGによる新規結核薬の迅速スクリーニング系の確立と実践 (2016)
  • Author(s): 尾関 百合子, 山口 雄大, Shymaa Enany, 五十嵐 雅之, 西内 由紀子, 岡 真優子, 岩本 朋忠, 小椋 義俊, 林 哲也, 立石 善隆, 西山 晃史, 松本 壮吉
  • Organizer: 第89回日本細菌学会
  • Place of Presentation: 大阪国際交流センター（大阪市天王寺区）
  • Year and Date: 2016-03-23
• [Presentation] 非結核性抗酸菌のバイオフィルム形成における Glycopeptidolipid の役割 (2016)
  • Author(s): 西内 由紀子, 戸谷 孝洋, 立石 善隆, 金子 幸弘, 松本 壮吉
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪市天王寺区）
  • Year and Date: 2016-03-23
• [Remarks] 新潟大学医学部細菌学
  • URL: https://www.med-niigatauniv-bacteriol.org/