| Project/Area Number |
15K21029
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
Neurophysiology / General neuroscience
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Nakashima Ai 東京大学, 大学院薬学系研究科(薬学部), 特任助教 (60706331)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 嗅覚 / 神経回路構築 / カルシウムイメージング / 嗅覚系 / 神経回路形成 / シグナル / 嗅細胞 / 神経活動 / 回路構築 / 軸索投射 |
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| Outline of Final Research Achievements |
In the mouse olfactory system, olfactory receptors (ORs) regulate a combinatorial expression of axon-sorting molecules at OSN axon termini through spontaneous neural activity. However, it remains unclear how different ORs generate different expression patterns of axon-sorting molecules. Here we have applied genetic approaches to explore regulatory mechanisms underlying their expressions. We conducted Calcium imaging using GCaMP6f sensor to monitor spontaneous activities of developing olfactory neurons. It was found that OSNs expressing the same OR exhibited similar patterns of calcium transients. However, cross-correlation analysis revealed that calcium events between OSNs expressing the same OR were not synchronized. These results indicate that temporal patterns of spontaneous activity, which are correlated with expressed OR types, are important to generate the combinatorial expressions of axon-sorting molecules for the OR-specific axon sorting.
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