Development of the method for evaluation of lipid absorption and gut immunology by using the mesenteric lymph cannulated rat model.
| Project/Area Number |
15K21037
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
Food science
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 腸間膜リンパ管 / カニュレーション / 免疫機能 / 脂質吸収 |
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| Outline of Final Research Achievements |
To elucidate the food-intake dependent relationship between lipid absorption and gut immunology, we developed the mesenteric lymph cannulated rat model. The all experiment was carried out under continuous isoflurane anesthesia. The lymph was collected from one hour before the administration of food sample (distilled water or cocoa butter) to four hours later of the administration. The lymph flow was increased in a short period after the administration of distilled water, however in the case of cocoa butter, it was increased 3-4hr later of the administration. It was not observed a big change in the ratio of T cell and B cell of the collected lymph. Interestingly, the IL-22 concentration of the collected lymph was 10 to 20 times higher than that of serum plasma. To elucidate the significance of IL-22 concentration in the lymph, further experiment will be needed.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance.2015
Author(s)
Zolla V, Nizamutdinova IT, Scharf B, Clement CC, Maejima D, Akl T, Nagai T, Luciani P, Leroux JC, Halin C, Stukes S, Tiwari S, Casadevall A, Jacobs WR Jr, Entenberg D, Zawieja DC, Condeelis J, Fooksman DR, Gashev AA, Santambrogio L
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Journal Title
Aging Cell
Volume: 14（4）
Issue: 4
Pages: 582-594
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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