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High-throughput analysis of olfactory receptor repertoire by using the automated single-cell analysis and isolation system

Research Project

Project/Area Number 15K21068
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Cell biology
Research InstitutionOsaka University

Principal Investigator

Yoshimoto Nobuo  大阪大学, 産業科学研究所, 特任准教授 (80467612)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords1細胞解析 / 嗅覚細胞 / 嗅覚受容体 / 匂い分子 / 嗅覚受容体レパトア / バイオセンサー / 全自動1細胞解析単離装置 / タイムラプスサイトメトリー
Outline of Final Research Achievements

A cell array consisting of 200,000 olfactory epithelial cells was formed on a microchamber array chip enabling cell alignment. After installing this cell array chip in the automated single-cell analysis and isolation system equipped with a perfusion system, the cells were subjected to time-lapse cytometry. Olfactory cells which transiently responded to stimulation of specific odor molecules were identified, and then the desired olfactory cells were isolated at single cell level. Each isolated single cell was subjected to genetic analysis to identify a combination of olfactory receptor groups that bind odor molecules specifically.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (26 results)

All 2016 2015 Other

All Journal Article (15 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 15 results,  Open Access: 13 results,  Acknowledgement Compliant: 5 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (3 results) Remarks (1 results)

  • [Journal Article] High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells2016

    • Author(s)
      Nobuo Yoshimoto, Shun'ichi Kuroda
    • Journal Title

      Methods in Molecular Biology pp

      Volume: 1487 Pages: 35-52

    • DOI

      10.1007/978-1-4939-6424-6_3

    • ISBN
      9781493964222, 9781493964246
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cellular uptake of hepatitis B virus envelope L particles is independent of sodium taurocholate cotransporting polypeptide, but dependent on heparan sulfate proteoglycan2016

    • Author(s)
      Masaharu Somiya, Qiushi Liu, Nobuo Yoshimoto, Masumi Iijima, Kenji Tatematsu, Tadashi Nakai, Toshihide Okajima, Kazuyuki Kuroki, Keiji Ueda, Shun’ichi Kuroda
    • Journal Title

      Virology

      Volume: 497 Pages: 23-32

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RBM20 and RBM24 cooperatively promote the expression of short enh splice variants2016

    • Author(s)
      Jumpei Ito, Masumi Iijima, Nobuo Yoshimoto, Tomoaki Niimi, Shun'ichi Kuroda, Andres D. Maturana
    • Journal Title

      FEBS Letter

      Volume: 590 Issue: 14 Pages: 2262-2274

    • DOI

      10.1002/1873-3468.12251

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mutational analysis of hepatitis B virus pre-S1 (9-24) fusogenic peptide2016

    • Author(s)
      Qiushi Liu, Masaharu Somiya, Naohiko Shimada, Wakako Sakamoto, Nobuo Yoshimoto, Masumi Iijima, Kenji Tatematsu, Tadashi Nakai, Toshihide Okajima, Atsushi Maruyama, Shun'ichi Kuroda
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 474 Issue: 2 Pages: 406-412

    • DOI

      10.1016/j.bbrc.2016.04.125

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Scaffold protein enigma homolog 1 overcomes the repression of myogenesis activation by inhibitor of DNA binding 22016

    • Author(s)
      Nakatani M. Ito J. Koyama R. Iijima M. Yoshimoto N. Niimi T. Kuroda S. Maturana AD
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 0 Issue: 2 Pages: 0-0

    • DOI

      10.1016/j.bbrc.2016.04.119

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Bio-nanocapsule-based scaffold improves the sensitivity and ligand-binding capacity of mammalian receptors on the sensor chip2016

    • Author(s)
      Iijima, M., Yoshimoto, N., Niimi, T., Maturana, A.D., Kuroda, S.
    • Journal Title

      Biotechnology journal

      Volume: 印刷中 Issue: 6 Pages: 805-813

    • DOI

      10.1002/biot.201500443

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bio-nanocapsules displaying various immunoglobulins as an active targeting-based drug delivery system2016

    • Author(s)
      Kenji Tatematsu, Masumi Iijima, Nobuo Yoshimoto, Tadashi Nakai, Toshihide Okajima, Shun’ichi Kuroda
    • Journal Title

      Acta Biomaterialia

      Volume: 35 Pages: 238-247

    • DOI

      10.1016/j.actbio.2016.02.010

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cytokine-dependent activation of the JAK-STAT pathway in Saccharomyces cerevisiae2016

    • Author(s)
      Yoshimoto, N., Ikeda, Y., Tatematsu, K., Iijima, M., Nakai, T., Okajima, T., Tanizawa, K., Kuroda, S.
    • Journal Title

      Biotechnology and Bioengineering

      Volume: 印刷中 Issue: 8 Pages: 1796-1804

    • DOI

      10.1002/bit.25948

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deciphering the Receptor Repertoire Encoding Specific Odorants by Time-Lapse Single-Cell Array Cytometry2016

    • Author(s)
      Suzuki M, Yoshimoto N, Shimono K, Kuroda S
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 19934-19934

    • DOI

      10.1038/srep19934

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] High-throughput analysis of mammalian receptor tyrosine kinase activation in yeast cells2016

    • Author(s)
      Yoshimoto N, Kuroda S
    • Journal Title

      Methods in Molecular Biology

      Volume: 9999

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mapping the heparin-binding site of the osteoinductive protein NELL1 by site-directed mutagenesis.2015

    • Author(s)
      Takahashi, K., Imai, A., Iijima, M., Yoshimoto, N., Maturana, A. D., Kuroda, S., and Niimi, T.
    • Journal Title

      FEBS Letters

      Volume: 589 Issue: 24PartB Pages: 4026-4032

    • DOI

      10.1016/j.febslet.2015.11.032

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Scaffold protein enigma homolog activates CREB whereas a short splice variant prevents CREB activation in cardiomyocytes2015

    • Author(s)
      Ito J, Iijima M, Yoshimoto N, Niimi T, Kuroda S, Maturana AD
    • Journal Title

      Cellular Signaling

      Volume: 27 Issue: 12 Pages: 2425-2433

    • DOI

      10.1016/j.cellsig.2015.09.007

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Virosomes of hepatitis B virus envelope L proteins containing doxorubicin: synergistic enhancement of human liver-specific antitumor growth activity by radiotherapy2015

    • Author(s)
      Liu Q, Jung J, Somiya M, Iijima M, Yoshimoto N, Niimi T, Maturana AD, Shin SH, Jeong SY, Choi EK, Kuroda S
    • Journal Title

      International Journal of Nanomedicine

      Volume: 10 Pages: 4159-4172

    • DOI

      10.2147/ijn.s84295

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Intracellular trafficking of bio-nanocapsule-liposome complex: Identification of fusogenic activity in the pre-S1 region of hepatitis B virus surface antigen L protein2015

    • Author(s)
      Somiya M, Sasaki Y, Matsuzaki T, Liu Q, Iijima M, Yoshimoto N, Niimi T, Maturana AD, Kuroda S
    • Journal Title

      Journal of Controlled Release

      Volume: 212 Pages: 10-18

    • DOI

      10.1016/j.jconrel.2015.06.012

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] One-step scalable preparation method for non-cationic liposomes with high siRNA content2015

    • Author(s)
      Somiya M, Yamaguchi K, Liu Q, Niimi T, Maturana AD, Iijima M, Yoshimoto N, Kuroda S
    • Journal Title

      International Journal of Pharmaceutics

      Volume: 490 Issue: 1-2 Pages: 316-323

    • DOI

      10.1016/j.ijpharm.2015.05.072

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Decipherment of olfactory receptor repertoire by using an automated single-cell analysis and isolation system2016

    • Author(s)
      Nobuo Yoshimoto, Masato Suzuki, Akiko Kida, Ken Shimono, Shun'ichi Kuroda
    • Organizer
      The 20th ISIR International Symposium
    • Place of Presentation
      大阪府大阪市
    • Year and Date
      2016-12-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞表層FIAとイムノチャンバーを用いる1細胞育種法の開発2016

    • Author(s)
      良元伸男、黒田俊一
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山県富山市
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Decipherment of olfactory receptor repertoire by using an automated single-cell analysis and isolation system equipped with real-time calcium imaging device2015

    • Author(s)
      Nobuo Yoshimoto
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Honolulu (USA)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 全自動1細胞解析単離装置を用いる1細胞育種とその応用技術2015

    • Author(s)
      良元伸男、木田晶子、黒田俊一
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 全自動1細胞解析単離装置による1細胞育種と様々な応用成果2015

    • Author(s)
      良元伸男
    • Organizer
      2015年度三賞受賞者合同発表会 in BioJapan2015
    • Place of Presentation
      パシフィコ横浜アネックスホール(神奈川県横浜市)
    • Year and Date
      2015-10-14
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 全自動1細胞解析単離装置を用いる優良細胞の1細胞育種2015

    • Author(s)
      良元伸男、木田晶子、黒田俊一
    • Organizer
      第28回 日本動物細胞工学会2015年度大会 (JAACT2015)
    • Place of Presentation
      東北大学 片平さくらホール(宮城県仙台市)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒト化抗体高分泌CHO細胞の1細胞スクリーニング法の開発2015

    • Author(s)
      木田晶子、良元伸男、黒田俊一
    • Organizer
      第28回 日本動物細胞工学会2015年度大会 (JAACT2015)
    • Place of Presentation
      東北大学 片平さくらホール(宮城県仙台市)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Research-status Report
  • [Book] AROMA RESEARCH No.67 「特定の匂い分子に応答する嗅覚受容体群の網羅的取得法」2016

    • Author(s)
      良元伸男、黒田俊一
    • Total Pages
      6
    • Publisher
      フレグランスジャーナル
    • Related Report
      2016 Annual Research Report
  • [Book] バイオサイエンスとインダストリー「【解説】1細胞育種を実現する全自動1 細胞解析単離装置の開発」2016

    • Author(s)
      良元伸男、黒田俊一
    • Total Pages
      4
    • Publisher
      バイオインダストリー協会
    • Related Report
      2015 Research-status Report
  • [Book] ファインケミカルシリーズ 抗体医薬における細胞構築・培養・ダウンストリームのすべて「第5章 1細胞育種を実現する全自動1細胞解析単離措置の開発」2015

    • Author(s)
      良元伸男、黒田俊一
    • Total Pages
      11
    • Publisher
      シーエムシー出版
    • Related Report
      2015 Research-status Report
  • [Remarks] 生体分子反応科学研究室

    • URL

      http://www.sanken.osaka-u.ac.jp/labs/smb/index.html

    • Related Report
      2016 Annual Research Report 2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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