Functional analysis of photosynthetic proteins by photoelectrochemistry-quartz crystal microbalance simultaneous measurement
| Project/Area Number |
15K21077
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
Polymer chemistry
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
Kondo Masaharu 名古屋工業大学, 工学(系)研究科(研究院), 助教 (20571219)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 水晶発振子 / 水晶発振子マイクロバランス法 / 同時計測 / 生体関連高分子 / 光合成色素－タンパク質 / 光電気化学 / タンパク質 / 光合成 |
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| Outline of Final Research Achievements |
In this study, we immobilized the photosynthetic reaction protein LH1-RC from a photosynthetic bacterium on a quartz crystal microbalance (QCM) sensor chip to observe the adsorption/desorption of molecules that exchanges electrons with LH1-RC by simultaneously measurements, QCM and photocurrent measurements. When the LH1-RC was immobilized on a QCM sensor chip surface-modified with an amino group and simultaneous measurement experiments were conducted, the photocurrent density was low because the amount of LH1-RC immobilized was very small. Since the photocurrent response was buried in the noise to the electrochemical measuring instrument, the evaluation in the simultaneous measurement system could not be performed with a sufficient signal. In a system using a conductive oxide substrate instead of a gold substrate, an increase in photocurrent density was confirmed by changing the concentration of electron acceptor; water-soluble ubiquinone and electron donor; cytochrome c.
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| Academic Significance and Societal Importance of the Research Achievements |
生物が行なう光合成反応では、光エネルギーを利用して電子を移動させる(光誘起電流を発生させる)反応中心RCが、電子受容体・供与体の分子と協同的にはたらくことで、高効率の光誘起電子移動を実現している。本研究では、RCとコアアンテナタンパク質(LH1)との複合体LH1-RCを水晶発振子上へ分子配向を制御し、活性を保ったまま固定化し、電気化学計測による光誘起電流とLH1-RCと電子の授受をする分子のLH1-RCへの吸脱着による重量変化をQCMの同時計測により基板上に固定化されたLH1-RCの機能・物性の評価を行なう。この評価で得られた知見から、生体高分子を用いた光水素生産デバイスの構築を目指す。
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Report
(5 results)
Research Products
(7 results)
