Establishment of novel pluripotent stem cells using genetically modified cynomolgus ES and iPS cells.
| Project/Area Number |
15K21080
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
General medical chemistry
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | iPS細胞 / ES細胞 / カニクイザル / トランスジェニック動物 / ナイーブ化 / ゲノム編集 / CRISPR/Cas9 |
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| Outline of Final Research Achievements |
Although transgenic technologies in non-human primates are useful for production of disease models and preclinical studies, only few reports have been published. In this study, we tried to generate transgenic animals and stem cells efficiently in cynomolgus monkeys. By using lentiviral vectors, we generated transgenic cynomolgus monkeys that express EGFP throughout the whole body for the first time. Additionally, we established a novel basic technology for generating high-quality ES/iPS cells in non-rodent species by using a CRISPR/Cas9 reporter system. We propose that these technologies can be applied to the medical industry in the future.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Flexible adaptation of male germ cells from female iPS cells of endangered Tokudaia osimensis2017
Author(s)
Honda A., Choijookhuu N., Izu H., Kawano Y., Inokuchi M., Honsho K., Lee A., Nabekura H., Ohta H., Tsukiyama T., Ohinata Y., Kuroiwa A., Hishikawa Y., Saitou M., Jogahara T., Koshimoto C.
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Journal Title
Science Advances
Volume: 印刷中
Related Report
Peer Reviewed / Open Access
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[Journal Article] Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body2016
Author(s)
Seita Y, Tsukiyama T, Iwatani C, Tsuchiya H, Matsushita J, Azami T, Okahara J, Nakamura S, Hayashi Y, Hitoshi S, Itoh Y, Imamura T, Nishimura M, Tooyama I, Miyoshi H, Saitou M, Ogasawara K, Sasaki E, Ema M
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Journal Title
Scientific Reports
Volume: 2
Issue: 1
Pages: 24868-24868
DOI
NAID
Related Report
Peer Reviewed / Open Access
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