Role of cohesin in T cell development
| Project/Area Number |
15K21083
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
General medical chemistry
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | T細胞分化 / エピジェネティクス / 染色体高次構造 / 染色体構造 / 遺伝子再構成 |
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| Outline of Final Research Achievements |
We generated Smc3 conditional knockout (cKO) mice, where the Smc3 locus could be deleted in various stages of T-lineages, and found that the cohesin is not essential for rearrangements of TCRb locus. Our data
shows that transcription factor E2A form foci in the nuclei of DN thymocytes, and that Vb regions come close to DJb regions near the E2A foci before the rearrangements of TCRb locus. These results suggest that E2A controls TCRb rearrangements by bringing Vb close to DJb on the E2A foci in DN thymocytes.
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Report
(4 results)
Research Products
(2 results)
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[Journal Article] STAT5 Orchestrates Local Epigenetic Changes for Chromatin Accessibility and Rearrangements by Direct Binding to the TCRγ Locus.2015
Author(s)
Wagatsuma K, Tani-Ichi S, Liang B, Shitara S, Ishihara K, Abe M, Miyachi H, Kitano S, Hara T, Nanno M, Ishikawa H, Sakimura K, Nakao M, Kimura H, Ikuta K.
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Journal Title
J Immunol.
Volume: 195
Issue: 4
Pages: 1804-14
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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