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Lipid-bilayer-assisted self-assembly of DNA origami arrays and their functionalization

Research Project

Project/Area Number 15K21093
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nanobioscience
Nanostructural chemistry
Research InstitutionTohoku University (2016)
Kyoto University (2015)

Principal Investigator

Suzuki Yuki  東北大学, 学際科学フロンティア研究所, 助教 (50636066)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsDNAナノテクノロジー / DNAオリガミ / 脂質二分子膜 / 自己集合化 / 自己組織化 / ボトムアップ技術 / 原子間力顕微鏡 / 人工脂質二分子膜
Outline of Final Research Achievements

In this study, we established a method to self-assemble DNA origami nanostructures into two-dimensional crystalline structures on planar lipid bilayer membranes. By changing the shape of DNA nanostructures and designing interactions between the DNA nanostructures, various two-dimensional crystalline structures were constructed. The self-assembled structures can be modified with heterogeneous molecules and be used as a scaffold for periodic arrangement of protein molecules and other nanostructures.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 6 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures2015

    • Author(s)
      Yuki Suzuki, Masayuki Endo, Hiroshi Sugiyama
    • Journal Title

      Nature Communications

      Volume: 6 Issue: 1 Pages: 8052-8052

    • DOI

      10.1038/ncomms9052

    • NAID

      120005649314

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mimicking membrane-related biological events by DNA origami nanotechnology2015

    • Author(s)
      Yuki Suzuki, Masayuki Endo, Hiroshi Sugiyama
    • Journal Title

      ACS Nano

      Volume: 9 Issue: 4 Pages: 3418-3420

    • DOI

      10.1021/acsnano.5b01723

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Self-assembly of two-dimensional DNA origami lattices on lipid membranes2017

    • Author(s)
      Yuki Suzuki, Masayuki Endo, Hiroshi Sugiyama, Ibuki Kawamata, Satoshi Murata
    • Organizer
      14th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices (FNANO17)
    • Place of Presentation
      Snowbird(Utah, U.S.A.)
    • Year and Date
      2017-04-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Organizing DNA origami components into crystalline structures at the lipid/aqueous solution interface2016

    • Author(s)
      Yuki Suzuki
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Organizing three-dimensional DNA origami objects into crystalline structures having periodic cavities2016

    • Author(s)
      Yuki Suzuki, Ibuki Kawamata, Satoshi Murata
    • Organizer
      The 43rd International Symposium on Nucleic Acids Chemistry (ISNAC2016)
    • Place of Presentation
      熊本大学(熊本県・熊本市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Filling cavities of lipid-bilayer-supported two-dimensional DNA origami arrays2016

    • Author(s)
      Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo
    • Organizer
      The 22nd International Conference on DNA Computing and Molecular Programming (DNA22)
    • Place of Presentation
      Munich (Germany)
    • Year and Date
      2016-09-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂質膜上におけるDNAオリガミナノ構造体の二次元自己集合化2016

    • Author(s)
      鈴木 勇輝, 遠藤 政幸,杉山 弘
    • Organizer
      日本顕微鏡学会第72回学術講演会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2016-06-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Lipid-bilayer-assisted 2D self-assembly of DNA origami nanostructures2015

    • Author(s)
      Yuki Suzuki, Masayuki Endo, Hiroshi Sugiyama.
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Honolulu (U.S.A.)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] DNA origami nanotechnology at membrane-solution interfaces2015

    • Author(s)
      Yuki Suzuki, Masayuki Endo, Hiroshi Sugiyama
    • Organizer
      「細胞を創る」研究会8.0
    • Place of Presentation
      大阪大学 (大阪府・吹田市)
    • Year and Date
      2015-11-12
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Formation of two-dimensional crystalline DNA origami lattices on lipid bilayer surfaces2015

    • Author(s)
      Yuki Suzuki, Masayuki Endo, Hiroshi Sugiyama
    • Organizer
      The 42th International Symposium on Nucleic Acids Chemistry (ISNAC 2015)
    • Place of Presentation
      Himeji (Japan)
    • Year and Date
      2015-09-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures2015

    • Author(s)
      Yuki Suzuki, Masayuki Endo, Hiroshi Sugiyama
    • Organizer
      21st International Conference on DNA Computing and Molecular Programming (DNA21)
    • Place of Presentation
      Boston/Cambridge (U.S.A.)
    • Year and Date
      2015-08-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] DNAオリガミの2次元自己集合化に成功 -ナノ~マイクロ空間での自在な分子配置も可能に-

    • URL

      http://www.kyoto-u.ac.jp/ja/research/research_results/2015/150827_1.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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