Expanding human iPS cell-derived alveolar type II cells and their application to disease modeling of hereditary pulmonary fibrosis
| Project/Area Number |
15K21114
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
Respiratory organ internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
Gotoh Shimpei 京都大学, 医学研究科, 准教授 (50747219)
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| Research Collaborator |
HOTTA Akitsu
ASAKA Isao
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 再生医学 / iPS細胞 / 呼吸器 / 肺線維症 / II型肺胞上皮細胞 / 分化 |
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| Outline of Final Research Achievements |
Efficient generation and expansion of human iPS cell-derived alveolar type II cells was achieved. The rate of SFTPC+ cells per total epithelial cells reached around 50 % and iPS cell-derived SFTPC+ cells were long-term cultured for more than three months. We applied these methods to disease modeling of Hermansky-Pudlak Syndrome. Abnormal lamellar body structure was observed in human iPS cell-derived alveolar type II cells. Human iPS cell-derived alveolar type II cells would be beneficial for disease modeling of pulmonary fibrosis.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Directed induction of functional multi-ciliated cells in proximal airway epithelial spheroids from human pluripotent stem cells.2016
Author(s)
Konishi S, Gotoh S, Tateishi K, Yamamoto Y, Korogi Y, Matsumoto H, Muro S, Hirai T, Ito I, Tsukita S, Mishima M.
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Journal Title
Stem Cell Reports
Volume: 6
Issue: 1
Pages: 18-25
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Long-term culture of alveolar epithelial type 2 cells derived from human induced pluripotent stem cells.2016
Author(s)
Yamamoto Y, Gotoh S, Korogi Y, Konishi S, Nagasaki T, Matsumoto H, Muro S, Hirai T, Ito I, Mishima M.
Organizer
International Society for Stem Cell Research 14th Annual Meeting
Place of Presentation
San Francisco, USA
Year and Date
2016-06-24
Related Report
Int'l Joint Research
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