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Evolution of the photosynthetic reaction center

Research Project

Project/Area Number 15K21122
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Structural biochemistry
Research InstitutionFukuoka University (2016-2017)
Osaka University (2015)

Principal Investigator

Mutoh Risa  福岡大学, 理学部, 助教 (10622417)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords光合成 / 反応中心 / X線結晶構造解析 / 電子スピン共鳴法 / 光合成反応中心 / X線結晶構造解析 / 界面活性剤
Outline of Final Research Achievements

Type 1 Reaction Center (RC) is the simplest structures among 4 kinds of RCs. Phototrophs are classified into two groups, oxygenic phototrophs and anaerobic phototrophs, and they have each RC. The structural and functional relationships in type 1 RCs remain poorly understood because of the difficulty with sample preparations due to their highly fragile properties against oxygenic air. I resolved the crystal structure of Type 1 RC at 3.2 Å resolution and found one new peptide which function is unknown. The existence of quinone molecules in Type 1 RC was unknown for long time. I identified RC contains 0.8 molecule quinones and found the electron density of quinones in the crystal structure.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 9 results,  Open Access: 9 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] X-ray crystallographic and high-speed AFM studies of peroxiredoxin 1 from Chlamydomonas reinhardtii2018

    • Author(s)
      Charoenwattanasatien Ratana、Tanaka Hideaki、Zinzius Karen、Hochmal Ana K.、Mutoh Risa、Yamamoto Daisuke、Hippler Michael、Kurisu Genji
    • Journal Title

      Acta Crystallographica Section F Structural Biology Communications

      Volume: 74 Issue: 2 Pages: 86-91

    • DOI

      10.1107/s2053230x17018507

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamics and energetics of cyanobacterial photosystem I:ferredoxin complexes in different redox states2017

    • Author(s)
      Setif Pierre、Mutoh Risa、Kurisu Genji
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Bioenergetics

      Volume: 1858 Issue: 7 Pages: 483-496

    • DOI

      10.1016/j.bbabio.2017.04.001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association of ferredoxin:NADP+ oxidoreductase with the photosynthetic apparatus modulates electron transfer in Chlamydomonas reinhardtii.2017

    • Author(s)
      Mosebach, L., Heilmann, C., Mutoh, R., Gaebelein, P., Steinbeck, J., Happe, T., Ikegami, T., Hanke, G., Kurisu, G., Hippler, M.
    • Journal Title

      Photosynth. Res.

      Volume: 134 Issue: 3 Pages: 291-306

    • DOI

      10.1007/s11120-017-0408-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hyperfine Sublevel Correlation Spectroscopy Studies of Iron-Sulfur Cluster in Rieske Protein from Green Sulfur Bacterium Chlorobaculum tepidum2017

    • Author(s)
      Hiroki Nagashima, Hiraku Kishimoto, Risa Mutton, Naotaka Terashima, Hirozo Oh-Oka, Genji Kurisu, Hiroyuki Mino
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 121 Issue: 12 Pages: 2543-2553

    • DOI

      10.1021/acs.jpcb.6b12968

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gallium ferredoxin as a tool to study the effects of ferredoxin binding to photosystem I without ferredoxin reduction2017

    • Author(s)
      Mignée C., Mutoh R., Krieger-Liszkay A., Kurisu G., Sétif P.
    • Journal Title

      Photosynth Research

      Volume: - Issue: 3 Pages: 251-263

    • DOI

      10.1007/s11120-016-0332-0

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Circadian oscillations of KaiA-KaiC and KaiB-KaiC complex formations in an in vitro reconstituted KaiABC clock oscillator.2016

    • Author(s)
      Murakami R, Mutoh R, Ishii K, Ishiura M.
    • Journal Title

      Genes Cells.

      Volume: 21 Issue: 8 Pages: 890-900

    • DOI

      10.1111/gtc.12392

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast.2016

    • Author(s)
      Hochmal, A.K., Zinzius, K., Charoenwattanasatien, R., Gabelein, P., Mutoh, R., Tanaka, H., Schulze, S., Liu, G., Scholz, M., Nordhues, A., Offenborn, J.N., Petroutsos, D., Finazzi, G., Fufezan, C., Huang, K., Kurisu, G. Hippler, M.
    • Journal Title

      Nature Commun.

      Volume: 7 Issue: 1 Pages: 11847-11847

    • DOI

      10.1038/ncomms11847

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Bacterial β-Glucosidase Reveals the Structural and Functional Basis of Genetic Defects in Human Glucocerebrosidase 2 (GBA2)2016

    • Author(s)
      Charoenwattanasatien, R., Pengthaisong, S., Breen, I., Mutoh, R., Sansenya, S., Hua, Y., Tankrathok, A., Wu, L., Songsiriritthigul, C., Tanaka, H., Williams, S.J., Davies, G.J., Kurisu, G., Cairns, J.R.
    • Journal Title

      ACS Chem Biol.

      Volume: 11 Issue: 7 Pages: 1891-1900

    • DOI

      10.1021/acschembio.6b00192

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] X-ray Structure and Nuclear Magnetic Resonance Analysis of the Interaction Sites of the Ga-Substituted Cyanobacterial Ferredoxin2015

    • Author(s)
      Mutoh Risa, Muraki Norifumi, Shinmura Kanako, Kubota-Kawai Hisako, Lee Young-Ho, Marc M. Nowaczyk, Matthias Rögner, Hase Toshiharu, Ikegami Takahisa, Kurisu Genji
    • Journal Title

      Biochemistry

      Volume: 54 Issue: 39 Pages: 6052-6061

    • DOI

      10.1021/acs.biochem.5b00601

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Studies on structure-function relationships among the Rieske protein and Cytochromes in Green sulfer bacteria2018

    • Author(s)
      H. Kishimoto, R. Mutoh, H. Tanaka, G. Kurisu, H. Oh-oka
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Heterologous expression and in vivo maturation of green algal [FeFe] hydrogenase in the strictly anaerobic photosynthetic bacterium Chlorobaculum tepidum2017

    • Author(s)
      池田 祐輔、武藤 梨沙、波佐間 雄世、大岡 宏造、栗栖 源嗣、寺内 一姫、浅井 智広
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 時間分解EPRでとらえる光合成反応中心初期電荷分離の制御機構2017

    • Author(s)
      H. Mino, H. Tsukuno, R. Mutoh, H. Nagashima, Y. Kobori, G. Kurisu, H. Oh-oka
    • Organizer
      第55回生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] X-ray structure and NMR analysis of the electron transfer complex between Photosystem I and Ferredoxin2017

    • Author(s)
      H. Tanaka, H. Kubota-Kawai, R. Mutoh, S. Pierre, M. Nowaczyk, M. Rogner, T. Ikegami, G. Kurisu
    • Organizer
      第55回生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The dynamics of photosystem 2 and light-harvesting complex 2 in spinach grana membrane revealed by high-speed AFM2017

    • Author(s)
      R. Mutoh, T. Iida, D. Yamamoto
    • Organizer
      第55回生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] へリオバクテリア反応中心の初期電荷分離スピン相関解析ラジカル対の捕捉2017

    • Author(s)
      佃野弘幸、武藤梨沙、栗栖源嗣、大岡宏造、三野広幸
    • Organizer
      第56回電子スピンサイエンス学会年会(SEST2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 緑色硫黄細菌のRieskeタンパク質可溶性ドメインの構造と相互作用解析2017

    • Author(s)
      H. Kishimoto, R. Mutoh, H. Tanaka, G. Kurisu, H. Oh-oka
    • Organizer
      第8回光合成学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analyses of transient absorption changes and low-temperature fluorescence in the photosynthetic reaction center of heliobacteria2016

    • Author(s)
      大岡 宏造、小島 理沙、浅井 智広、武藤 梨沙、栗栖 源嗣、伊藤 繁
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Chlorobaculum tepidum還元型 [2Fe-2S] Rieske clusterの磁気構造解析2016

    • Author(s)
      寺島尚貴1、長嶋宏樹、岸本拓、武藤梨沙、大岡宏造、栗栖源嗣、三野広幸
    • Organizer
      第55回電子スピンサイエンス学会年会(SEST2016)
    • Place of Presentation
      大阪市立大学杉本キャンパス
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
  • [Presentation] 時間分解EPRによるヘリオバクテリア反応中心での初期ラジカル対生成2016

    • Author(s)
      佃野 弘幸、武藤 梨沙、栗栖 源嗣、大岡 宏造、三野 広幸
    • Organizer
      第55回電子スピンサイエンス学会年会(SEST2016)
    • Place of Presentation
      大阪市立大学杉本キャンパス
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
  • [Presentation] Conformational Changes of Cyanobacterial Circadian Clock Proteins2016

    • Author(s)
      Risa Mutoh, Hiroyuki Mino, Masahiro Ishiura
    • Organizer
      The 4th Awaji International Workshop on Electron Spin Science & Technology: Biological and Materials Science
    • Place of Presentation
      淡路夢舞台(兵庫)
    • Year and Date
      2016-06-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ヘリオバクテリア由来の1型光合成反応中心の活性測定2016

    • Author(s)
      安田亜矢、武藤梨沙、大岡宏造、浅井智広、栗栖源嗣
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県盛岡市))
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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