| Project/Area Number |
15K21137
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
HOBRO ALISON 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (50622148)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Spectral Analysis / Molecular imaging / Raman / Imaging / Viruses / Molecular Imaging |
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| Outline of Final Research Achievements |
This project utilised Raman spectroscopy (a label-free, non-invasive measurement technique) to study the initial stages of viral infection. This involved trying to detect the presence of viral particles in solutions (such as serum), as well as measuring infected cells to understand how infection progresses and how cells respond to the presence of a virus. The effects of cell fixation and cell cycle on Raman images/spectra were investigated in order to characterise factors that may also affect the cells (so they are not attributed to the effects of the virus). Raman analysis of apoptosis, induced through the damage of major organelles, was also investigated to draw parallels to potential changes occurring during infection.
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