| Project/Area Number |
15K21150
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental conscious materials and recycle
Applied microbiology
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| Research Institution | Kobe University |
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Principal Investigator |
Sasaki Daisuke 神戸大学, 科学技術イノベーション研究科, 特命助教 (00650615)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 高温メタン発酵 / 組換え遺伝子 / 水平伝播 / 環境拡散 / 廃菌処理 / 廃棄物処理 / GFP遺伝子 / 組み換え遺伝子 / エネルギー回収 |
|---|
| Outline of Final Research Achievements |
The possibility of horizontal gene transfer and environmentally spread of an exogenous gene into microbial communities was investigated by introducing yeast which constantly expresses recombinant gene (green fluorescent protein) in thermophilic methane fermentation reactor. As a result of operation of the fermenter for a long period and molecular biological analysis and microbiological analysis, when dead or living yeast holding the recombinant gene were introduced into the reactor, the gene was immediately degraded. Therefore, it was revealed for the first time that neither horizontal gene transfer nor spread of exogenous gene occurs.
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