The role of Trans-fatty acids in deep venous thrombosis

• Project/Area Number: 15K21154
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine; Metabolomics
• Research Institution: Kobe University
• Principal Investigator: Hideto Nakajima 神戸大学, 医学研究科, 医学研究員 (70726992)
• Research Collaborator: TOH RYUJI 神戸大学, 医学研究科, 特命准教授 (50379418) ; ISHIDA TATSUEO 神戸大学, 医学研究科, 特命教授 (00379413)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 血栓 / トランス脂肪酸

Outline of Final Research Achievements

(1) Five percent of elaidic acid treatment increased the serum concentration of elaidic acid at almost 100-fold. This indicates oral elaidic acid treatment actually increase the circulating elaidic acid level and incorporated into the organs in mice.
(2) Afte the 4 weeks of treatment of elaidic acid or oleic acis as a control, we performed IVC ligation to induce deep venous thrombosis. Unfortunately, 6 mice died after the operation. We did not observe statistical significance, but there maybe a tendency that elaidic acid augments the DVT formation. We therefore add 10 more mice to have final conclusion.

Research Products

• [Journal Article] Excessive intake of trans fatty acid accelerates atherosclerosis through promoting inflammation and oxidative stress in a mouse model of hyperlipidemia. (2017)
  • Author(s): Monguchi T, Hara T, Hasokawa M, Nakajima H, Mori K, Toh R, Irino Y, Ishida T, Hirata KI, Shinohara M
  • Journal Title: J Cardiol Volume: 印刷中 Issue: 2 Pages: 121-127
  • DOI: 10.1016/j.jjcc.2016.12.012
  • Peer Reviewed
• [Journal Article] 2-Aminobutyric acid modulates glutathione homeostasis in the myocardium. (2016)
  • Author(s): Irino Y, Toh R, Nagao M, Mori T, Honjo T, Shinohara M, Tsuda S, Nakajima H, Satomi-Kobayashi S, Shinke T, Tanaka H, Ishida T, Miyata O, Hirata KI.
  • Journal Title: Sci Rep. Volume: - Issue: 1 Pages: 36749-36749
  • DOI: 10.1038/srep36749
  • NAID: 120005898711
  • Peer Reviewed / Open Access
• [Journal Article] β-Hydroxybutyrate elevation as a compensatory response against oxidative stress in cardiomyocytes. (2016)
  • Author(s): Nagao M, Toh R, Irino Y, Mori T, Nakajima H, Hara T, Honjo T, Satomi-Kobayashi S, Shinke T, Tanaka H, Ishida T, Hirata K.
  • Journal Title: Biochem Biophys Res Commun Volume: 475 Issue: 4 Pages: 322-328
  • DOI: 10.1016/j.bbrc.2016.05.097
  • Peer Reviewed