| Project/Area Number |
15K21172
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
Surgical dentistry
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 顎骨再生 / 生体材料 / 骨髄幹細胞 / iPS細胞 / 足場材料 / 顎骨再建 / 骨伝導能 / 骨芽細胞 / 口腔顎顔面外科 / 骨再生 / 間葉系幹細胞 / 3次元多孔質生体吸収性足場 / 骨髄由来間葉系幹細胞 / 骨形成 |
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| Outline of Final Research Achievements |
The bimaxillary boney and soft-tissue defects following oral-maxillofacial tumor or trauma could cause loss of esthetics and function. Although the reconstructive surgery using autologous tissue transfer should be applied, the surgical invasiveness is so severe. With this scientific grant, I tried to develop the innovative maxillofacial boney regenerative technique using feasible 3D-uHA/PDLLA bioactive scaffold and iPS cells. I first tried to induce human iPS cells into boney regenerative/osteogenic cells selectively, however, the technique couldn't be well established. Then, I applied human bone marrow derived-mesenchymal stem cells (hMSC) for 3D-uHA/PDLLA scaffold. First, the boney regenerative/osteogenic hMSC were isolated using FACS for grafting. These osteognic cells were transfered into 3D-uHA/PDLLA scaffold, and were grafted into the mandibular critical defects in animal models. The results obtained were feasible accelerating and inducing optimal boney regeneration.
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