| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In order to investigate whether hypoxia-selective nano DDS preparation shows neuroprotective effect under hypoxic conditions, an in vitro system using mouse hippocampal HT22 neurons was established. Exposure of HT22 neurons in a low oxygen incubator under 1% oxygen conditions for 6 hours resulted in a cell survival rate of 43%. In addition, thiopental as an anticonvulsant and nicorandil as a therapeutic agent for angina pectorally inhibited nerve cell death in hypoxic conditions in a concentration-dependent manner. From these results, it is considered that Two drugs which found neuroprotective effect in this study is useful for preparing hypoxia selective nano DDS formulations. In the future, we will investigate the efficacy by administering nano DDS formulated drug to cardiopulmonary arrest model rats and aim for development of post-resuscitation encephalopathy drugs.
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