Establishment of quantitative evaluation method of lymphatic transport function using in vivo imaging

• Project/Area Number: 15K21182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery; Cardiovascular surgery
• Research Institution: Okayama University
• Principal Investigator: Sugiyama Narushi 岡山大学, 医学部, 客員研究員 (80379776)
• Research Collaborator: Kimata Yoshihiro ; Yamada Kiyoshi
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: リンパ輸送機能 / 定量評価 / リンパ浮腫

Outline of Final Research Achievements

To quantify function of lymphatic transportation, several conditions were examined by measuring remaining IRDye PEG as a fluorescent reagent injected at mouse hind limb using in vivo imaging over time, but no significant results were obtained. It was suggested the possibility that quantitative evaluation of lymphatic transportation can be performed by administering Fluorescent Dextran as a fluorescent reagent to the rat hind limb and measuring the fluorescence intensity of the blood sample collected over time to evaluate the amount of Fluorescent Dextran flowing into the blood along with the lymphatic flow.

Academic Significance and Societal Importance of the Research Achievements

従来リンパ系の輸送機能を定量評価する検査が無かったが、新たな定量評価法の確立につながる結果が得られた。