Effects of a naturally occurring dioxin, 1,3,7-Tribromodibenzo-p-dioxin on the mysid transcriptome

• Project/Area Number: 15K21204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals; Environmental risk control and evaluation
• Research Institution: National Institute of Technology, Kumamoto College (2016-2017); Ehime University (2015)
• Principal Investigator: Hirano Masashi 熊本高等専門学校, 生物化学システム工学科, 准教授 (20554471)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 臭素化ダイオキシン / トランスクリプトーム / 甲殻類 / 海産無脊椎動物 / 環境化学物質 / 生態毒性 / RNA-seq

Outline of Final Research Achievements

Some of polybrominated dibenzo-p-dioxin congeners (PBDDs) including di-/triBDDs are ubiquitously detected in marine sediments and organisms. To understand the response to the PBDD exposure, we conducted RNA-seq analysis of the transcriptome in mysids treated with vehicle or 1,3,7-TriBDD. For comparison, the transcriptomes in mysids treated with TCDD, Kanechlor-500 (KC-500), and bisphenol A were also analyzed. The gene expression patterns showed that the transcriptome response to 1,3,7-TriBDD was different from those to chlorinated analogues, TCDD and KC-500. Heat map and K-mean cluster analyses revealed that myosin-related genes were markedly down-regulated by 1,3,7-TriBDD exposure, implying that 1,3,7-TriBDD might influence the behavior of mysid. These results suggest that PBDDs may have a potential to elicit adverse effects in the mysid through a distinct mechanism from other chemicals.

Research Products

• [Journal Article] 海産甲殻類アミに対する4-ノニルフェノール異性体の急性・亜慢性毒性影響 (2017)
  • Author(s): 内田雅也、平野将司、石橋弘志、冨永伸明、有薗幸司
  • Journal Title: 環境毒性学会誌 Volume: 20 Pages: 59-68
  • NAID: 130007833419
  • Peer Reviewed
• [Journal Article] Accumulation properties of polychlorinated biphenyl congeners in Yusho patients and prediction of their cytochrome P450-dependent metabolism by in silico analysis (2017)
  • Author(s): Hirakawa S, Miyawaki T, Hori T, Kajiwara J, Katsuki S, Hirano M, Yoshinouchi Y, Iwata H, Mitom C, Furue M
  • Journal Title: Environmental Science and Pollution Research International Volume: 1 Issue: 17 Pages: 1-9
  • DOI: 10.1007/s11356-017-9498-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Strain differences in the proteome of dioxin-sensitive and -resistant mice treated with 2,3,7,8-tetrabromodibenzo-p-dioxin (2017)
  • Author(s): Nguyen, H.T., Lauan Tsuchiya, M.C., Yoo, J., Iida, M., Agusa, T., Hirano, M., Kim, E.-Y., Miyazaki, T., Nose, M., Iwata, H.
  • Journal Title: Archives of Toxicology Volume: 4 Issue: 4 Pages: 1763-1782
  • DOI: 10.1007/s00204-016-1834-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transcriptional response of mysid crustacean, Americamysis bahia, is affected by subchronic exposure to nonylphenol. (2016)
  • Author(s): Uchida M*, Hirano M*, Ishibashi H, Kobayashi J, Kagami Y, Koyanagi A, Kusano T, Koga M, Arizono K.(*, Contributed equally)
  • Journal Title: Ecotoxicol Environ Saf. Volume: 133 Pages: 360-365
  • DOI: 10.1016/j.ecoenv.2016.07.037
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of aryl hydrocarbon receptor signaling pathways altered in TCDD- treated red seabream embryos by transcriptome analysis (2016)
  • Author(s): Midori Iida, Satoshi Fujii, Masaya Uchida, , Hiroshi Nakamura, Yoshihiro Kagami, Tetsuro Agusa, Masashi Hirano, Su-Min Bak, Eun-Young Kim, Hisato Iwata
  • Journal Title: Aquatic Toxicology Volume: 177 Pages: 156-170
  • DOI: 10.1016/j.aquatox.2016.05.014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ecological factors drive natural selection pressure of avian aryl hydrocarbon receptor 1 genotypes (2016)
  • Author(s): Hwang, J. H., Park, J. Y., Park, H. J., Bak, S. M., Hirano, M., Iwata, H., Park, Y. S. and Kim, E. Y.
  • Journal Title: Scientific Reports Volume: 6(27526) Issue: 1 Pages: 2045-2322
  • DOI: 10.1038/srep27526
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] In vitro and in silico evaluation of transactivation potencies of avian AHR1 and AHR2 by endogenous ligands: implications for the physiological role of avian AHR2 (2016)
  • Author(s): Kim, I.S., Hwang, J.H., Hirano, M., Iwata, H. and Kim, E.Y.
  • Journal Title: Comp. Biochem. Physiol. C. Volume: 187 Pages: 1-9
  • DOI: 10.1016/j.cbpc.2016.03.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] In vitro and in silico analyses for predicting hepatic cytochrome p450-dependent metabolic potencies of polychlorinated biphenyls in the Baikal seal (2015)
  • Author(s): Jean Yoo, Masashi Hirano, Hazuki Mizukawa, Kei Nomiyama, Tetsuro Agusa, Eun-Young Kim, Shinsuke Tanab, Hisato Iwata
  • Journal Title: Environmental Science and Technology Volume: 49 Issue: 24 Pages: 14588-14596
  • DOI: 10.1021/acs.est.5b03890
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ネオニコチノイド系農薬によるメダカ胚トランスクリプトームへの影響 (2017)
  • Author(s): 内田雅也、山口明美、平野将司、河野晋、石橋弘志、有薗幸司、冨永伸明
  • Organizer: 第20回環境ホルモン学会
• [Presentation] メダカ初期胚を用いたネオニコチノイドと代謝物の発生影響評価 (2017)
  • Author(s): 山口明美、平野将司、河野晋、石橋弘志、高橋圭介、加藤恵介、内田雅也、有薗幸司、冨永伸明
  • Organizer: 第20回環境ホルモン学会
• [Presentation] イヌ・アザラシ・マウスのエストロゲン受容体転写活性化能の種差を規定する分子機序 (2017)
  • Author(s): 芳之内結加、奥田博貴、平野将司、野見山桂、田辺信介、金恩英、岩田久人
  • Organizer: 第20回環境ホルモン学会
• [Presentation] Hepatic transcriptome profiles of rats exposed to bisphenol A during pregnancy: comparison with those of their offspring (2017)
  • Author(s): Hoa Thanh Nguyen、Kimika Yamamoto、Midori Iida、Tetsuro Agusa、Masashi Hirano、Mari Ochiai、Eun-Young Kim、Hisato Iwata
  • Organizer: 第20回環境ホルモン学会
• [Presentation] In silico試験系を用いたパラオキシ安息香酸エチルのエストロゲン作用の評価 (2017)
  • Author(s): 広田菜々、平野将司、石橋弘志、山元涼子、髙本亜希子、有薗幸司
  • Organizer: 第20回環境ホルモン学会
• [Presentation] EFFECTS OF A NATURALLY SYNTHESIZED DIOXIN, 1,3,7-TRIBROMODIBENZO-P-DIOXIN ON THE MYSID TRANSCRIPTOM (2017)
  • Author(s): Hirano, M., Uchida, M., Kusano, T., Arizono, A., Iwata, H.
  • Organizer: 19th Pollutant Responses in Marine Organisms
  • Int'l Joint Research
• [Presentation] IN SILICO AND IN VITRO SCREENING OF THE EFFECTS OF PPCPS ON MYSID ECDYSTEROID RECEPTOR SIGNALING (2017)
  • Author(s): Hirano, M., Ohno, Y., Iwata, H.
  • Organizer: 19th Pollutant Responses in Marine Organisms
  • Int'l Joint Research
• [Presentation] NEUROTOXICITY ASSESSMENT OF ENVIRONMENTAL CONTAMINATS WITH CHEMICALLY-INDUCED NEURONS OF DOLPHINS (2017)
  • Author(s): Ochiai, M., Nguyen, T.H., Kurihara, N., Hirano, M., Murakami, Y., Iwata, H.
  • Organizer: 19th Pollutant Responses in Marine Organisms
  • Int'l Joint Research
• [Presentation] STRUCTURE-ACTIVITY RELATIONSHIPS OF BAIKAL SEAL ESTROGEN RECEPTORS AND ENVIRONMENTAL POLLUTANTS (2017)
  • Author(s): Yoshinouchi, Y., Hirano, M., Nakata, H., Nimiyama, K., Tanabe, S., Kim, E.Y., Iwata, H.
  • Organizer: 19th Pollutant Responses in Marine Organisms
  • Int'l Joint Research
• [Presentation] PCBsのin vivo曝露によるビーグル犬の肝トランスクリプトームとメタボロームへの影響 (2016)
  • Author(s): 田村沙弥, 阿草哲郎, 平野将司, 江口哲史, 見野山桂, 田辺信介, 金恩英, 岩田久人
  • Organizer: 第19回環境ホルモン学会
  • Place of Presentation: 文科省研究交流センター(茨城県つくば市)
  • Year and Date: 2016-12-08
• [Presentation] IN VITRO AND IN SILICO APPROACHES FOR ASSESSING THE ACTIVATION OF BAIKAL SEAL ESTROGEN RECEPTORS BY OH-PCBS. (2016)
  • Author(s): Yoshinouchi Y, Hirano M, Nomiyama K, Tanabe S, Kim EY, Iwata H
  • Organizer: The 36th International Symposium on Halogenated Persistent Organic Pollutants, Dioxin 2016 symposium
  • Place of Presentation: Firenze(Italy)
  • Year and Date: 2016-08-28
  • Int'l Joint Research
• [Presentation] In silico and in vitro screening of the effects of PPCPs on mysid ecdysteroid receptor signaling (2015)
  • Author(s): Hirano, M., Ohno, Y., Iwata, H.
  • Organizer: 第18回環境ホルモン学会
  • Place of Presentation: 自治医科大学（栃木県・下野市薬師寺）
  • Year and Date: 2015-12-10
• [Presentation] Effects of prenatal exposure to bisphenol A on the liver transcriptome of rat offspring (2015)
  • Author(s): Nguyen, T.H., Yamamoto, K., Agusa, T., Hirano, M., Ochiai, M., Kim, E.Y. Iwata, H.
  • Organizer: 第18回環境ホルモン学会
  • Place of Presentation: 自治医科大学（栃木県・下野市薬師寺）
  • Year and Date: 2015-12-10
• [Presentation] OH-PCBsによるバイカルアザラシ エストロゲン受容体α・β転写活性化能の評価 (2015)
  • Author(s): 芳之内結加, 平野将司, 野見山桂, 田辺信介, 金恩英, 岩田久人
  • Organizer: 第18回環境ホルモン学会
  • Place of Presentation: 自治医科大学（栃木県・下野市薬師寺）
  • Year and Date: 2015-12-10
• [Presentation] トランスクリプトーム解析によるPCBsを曝露したイヌへの影響評価 (2015)
  • Author(s): 田村沙弥, Yoo Jean, 水川葉月, 阿草哲郎, 平野将司, 江口哲史, 見野山桂, 田辺信介, 金恩英, 岩田久人
  • Organizer: 第18回環境ホルモン学会
  • Place of Presentation: 自治医科大学（栃木県・下野市薬師寺）
  • Year and Date: 2015-12-10
• [Presentation] 鳥類の化学物質感受性を規定する分子機序 (2015)
  • Author(s): 岩田久人，平野将司，金恩英
  • Organizer: 第42回日本毒性学会学術年会，シンポジウム9 ~異物/薬物代謝の種差解明の最先端~
  • Place of Presentation: ホテル日航金沢（石川県・金沢市本町）
  • Year and Date: 2015-06-29
  • Invited
• [Presentation] Hepatic cytochrome P450-dependent metabolic potency of polychlorinated biphenyls in the Baikal seal (Pusa sibitica) (2015)
  • Author(s): Yoo, J., Hirano, M., Mizukawa, H., Nomiyama, K., Agusa, T., Kim, E.Y., Tanabe, S., Iwata, H.
  • Organizer: The 18th Pollutant Responses in Marine Organisms (PRIMO) Symposium
  • Place of Presentation: Trondheim, Norway
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Presentation] Hepatic proteome analysis of Baikal seals contaminated with dioxins and related compounds (2015)
  • Author(s): Lauan, M.C., Agusa, T., Hirano, M., Yoo, J., Kim, E.Y., Iwata, H.
  • Organizer: The 18th Pollutant Responses in Marine Organisms (PRIMO) Symposium
  • Place of Presentation: Trondheim, Norway
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Presentation] Ecological factors affecting natural selection of avian AhR1 genotypes (2015)
  • Author(s): Hwang, J.H., Park, H.J., Park, J.Y., Bak, S.M., Hirano, M., Iwata, H., Park, Y.S., Kim, E.Y.
  • Organizer: The 18th Pollutant Responses in Marine Organisms (PRIMO) Symposium
  • Place of Presentation: Trondheim, Norway
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Presentation] IN VITRO AND IN SILICO ASSESSMENT OF TRANSACTIVATION POTENCIES OF BAIKAL SEAL ESTROGEN RECEPTORS (2015)
  • Author(s): Yoshinouchi, Y., Shimizu, S., Lee, J.S., Hirano, M., Agusa, T., Suzuki, K.T., Nakata, H., Kim, E.Y., Iwata, H.
  • Organizer: The 18th Pollutant Responses in Marine Organisms (PRIMO) Symposium
  • Place of Presentation: Trondheim, Norway
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Presentation] バイカルアザラシ エストロゲン受容体を介したビスフェノール類によるアンタゴニスト作用の評価 (2015)
  • Author(s): 芳之内結加, 清水沙千子, 李鎭善, 平野将司, 鈴木賢一, 中田晴彦, 金恩英, 岩田久人
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター(北海道・札幌市白石区)
  • Year and Date: 2015-05-24