Screening of essential ICB associate factors for spermatogenesis to understand male infertility

• Project/Area Number: 15K21217
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science; Animal production science
• Research Institution: Kyushu University
• Principal Investigator: Iwamori Tokuko 九州大学, 医学研究院, 助教 (10711509)
• Research Collaborator: Kato Yuzuru ; Iwamori Naoki ; Matsumoto Masaki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生殖細胞間架橋 / 精子形成 / KIAA1210 / 精巣特異的細胞間結合 / Intercellular bridge / Topoisomerase2B / 生殖生物学 / 細胞間架橋 / RNA結合タンパク質

Outline of Final Research Achievements

Deletion of intercellular bridge (ICB), which connects all germ cells, caused male infertility. But the function of ICB still remains unknown. To understand the significance of ICB in spermatogenesis, its associating factors were identified and the interactions among each identified protein were comprehensively profiled. Specific antibodies to selected target proteins were developed and used to analyze their localization in testis. As a result, a novel Ectoplasmic Specialization (EPS) associating protein, KIAA1210, was identified. The knockout mouse of KIAA1210 was successfully produced and is under analyses. The results from analyses of KIAA1210 deficient mice will bring deep understandings of interactions between ICB and the other cell junction in spermatogenesis.

Research Products

• [Int'l Joint Research] Baylor College of Medicine(米国)
• [Journal Article] Identification of KIAA1210 as a novel X-chromosome-linked protein that localizes to the acrosome and associates with the ectoplasmic specialization in testes. (2017)
  • Author(s): Iwamori T, Iwamori N, Matsumoto M, Ono E, Matzuk MM.
  • Journal Title: Biol Reprod Volume: 96 Issue: 2 Pages: 469-477
  • DOI: 10.1095/biolreprod.116.145458
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MRG15 is required for pre-mRNA splicing and spermatogenesis. (2016)
  • Author(s): Iwamori N, Tominaga K, Sato T, Riehle K, Iwamori T, Ohkawa Y, Coarfa C, Ono E, Matzuk MM.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 113 Issue: 37
  • DOI: 10.1073/pnas.1611995113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Astrocytic and neuronal localization of kunurenine aminotransferase-2 in the adult mouse brain. (2016)
  • Author(s): Heredi J, Berko AM, Jankovics F, Iwamori T, Iwamori N, Ono E, Horvath S, Kis Z, Toldi J, Vecsei L, Gellert L.
  • Journal Title: Brain Struct Funct Volume: 印刷中 Issue: 4 Pages: 1663-1672
  • DOI: 10.1007/s00429-016-1299-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay (2016)
  • Author(s): Uchida A, Kishi K, Aiyama Y, Miura K, Takase HM, Suzuki H, Kanai-Azuma M, Iwamori T, Kurohmaru M, Tsunekawa N, Kanai Y
  • Journal Title: Biochem Biophys Res Commun Volume: 476 Issue: 4 Pages: 546-552
  • DOI: 10.1016/j.bbrc.2016.05.160
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 精巣特異的細胞間結合ICB, ES,BTBの連携作用に機能するタンパク質 (2017)
  • Author(s): 岩森督子、岩森巨樹、松本雅記、小野悦郎、Martin M. Matzuk
  • Organizer: 生命科学系学会合同年次大会(第40回日本分子生物学会大会)
• [Presentation] A novel X-chromosome-linked protein, KIAA1210, localizes to the acrosome and associates with the ectoplasmic specialization in testes. (2017)
  • Author(s): Tokuko Iwamori , Yuzuru Kato, Naoki Iwamori, Masaki Matsumoto, Yumiko Saga, Etsuro Ono and Martin M. Matzuk
  • Organizer: 4th World Congress of Reproductive Biology （第110回日本繁殖生物学会大会）
• [Presentation] 精巣特異的アクチン関連細胞間結合Ectoplasmic specializatioとアクロソームに局在する新規タンパク質の同定 (2016)
  • Author(s): 岩森督子、岩森巨樹、松本雅記、小野悦郎、Martin M. Matzuk
  • Organizer: 九州実験動物研究会
  • Place of Presentation: 北九州、産業医科大学
  • Year and Date: 2016-10-29
• [Presentation] MRG15はスプライシング制御を介して精子形成に必須の役割を果たす (2016)
  • Author(s): 岩森巨樹、富永薫、佐藤哲也、岩森督子、大川恭行、小野悦郎、Martin M. Matzuk
  • Organizer: 九州実験動物研究会
  • Place of Presentation: 北九州、産業医科大学
  • Year and Date: 2016-10-29
• [Presentation] Identification and functional analysis of novel protein on testis specific actin related cell-cell junction, ES (Ectoplasmic Specialization) (2015)
  • Author(s): Tokuko Iwamori, Naoki Iwamori, Masaki Matsumoto, Etsuro Ono, Martin M. Matzuk
  • Organizer: 第３８回 日本分子学生物学会年会
  • Place of Presentation: 兵庫県 神戸ホートアイランド
  • Year and Date: 2015-12-01