The analysis of the mechanisms of HERV-K expression
| Project/Area Number |
15K21242
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
Genetics/Chromosome dynamics
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| Research Institution | Kumamoto University |
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Principal Investigator |
MONDE KAZUAKI 熊本大学, 大学院生命科学研究部(医), 助教 (70516137)
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| Research Collaborator |
Sawa Tomohiro 熊本大学, 大学院生命科学研究部, 教授 (30284756)
MAEDA Yosuke 熊本大学, 大学院生命科学研究部, 准教授 (30284764)
ONO Akira University of Michigan, Department of Microbiology and Immunology, Associate Professor
Nagashima Kunio Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research・Electron Microscopy Laboratory, Professor
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | HERV-K / レトロトランスポゾン / 内在性レトロウイルス / HIV-1 / Gag / Sox2 / SOX2 / iPS / ヒト内在性レトロウイルス |
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| Outline of Final Research Achievements |
Human endogenous retroviruses (HERVs), the remnants of ancient retroviral infections, constitute about 8% of human genomic DNA. HERVs are costitutively transcribed in primordial germ cells. Eventually, HERV-K transcription is epigenetically silenced in somatic cells except for in pathological contexts. HERV-K expression is induced by HIV-1-infection in T cells. We found that HERV-K interferes the HIV-1 replication. HERV-K Gag MA, CA and NC are important for reduction of HIV-1 release and infectivity. It is conceivable that HERVs, which have adapted to human for a long time, might have protected the host from the threat of exogenous retroviruses. Our further analysis revealed that Sox2 is essential for the transcription of HERV-K. Interestingly, HERV-K had a retrotransposon activity and moved on the host genomes for a long-term culturing. It suggests that HERV-K is likely to be epigenetically regulated and play a role for development of embryo in short-term period of early development.
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Report
(4 results)
Research Products
(17 results)
